Name: Recombinant Mouse Sulfotransferase 1A1 (Sult1a1)
Brand: CUSABIO
SKU: CSB-EP022933MO

Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

Sult1a1

Uniprot No.

P52840

Research Area

Signal Transduction

Alternative Names

Sult1a1; St1a1; Stp; Stp1; Sulfotransferase 1A1; ST1A1; EC 2.8.2.1; Aryl sulfotransferase; Phenol sulfotransferase; Phenol/aryl sulfotransferase; mSTp1; ST1A4; Sulfokinase

Species

Mus musculus (Mouse)

Source

E.coli

Expression Region

1-291aa

Target Protein Sequence

MEPLRKPLVPVKGIPLIKYFAETMEQLQNFTAWPDDVLISTYPKSGTNWMSEIMDMIYQGGKLDKCGRAPVYARIPFLEFSCPGVPPGLETLKETPAPRIIKTHLPLSLLPQSLLDQKIKVIYVARNAKDVVVSYYNFYKMAKLHPDPGTWESFLENFMDGKVSYGSWYQHVKEWWELRRTHPVLYLFYEDMKENPKREIKKILEFLGRSLPEETVDLIVHHTSFKKMKENPMANYTTIPTEVMDHTIYPFMRKGTIGDWKNTFTVAQSEHFDAHYAKLMTGCDFTFRCQI
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

54.0kDa

Protein Length

Full Length

Tag Info

N-terminal 10xHis-SUMO-tagged and C-terminal Myc-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

To produce recombinant Mouse Sult1a1 protein, a well-established recombinant DNA technology is the key. A DNA template of Sult1a1 was constructed with N-terminal 10xHis-SUMO tag & C-terminal Myc tag using the technique. Once the template was made, the recombinant Mouse Sult1a1 protein could be produced with it efficiently. CUSABIO has built a strict QC system to ensure quality. The expression region is 1-291aa of the Mouse Sult1a1. The purity of this recombinant is 90% determined by SDS-PAGE.

Sult1a1 is a protein coding gene that encodes Sulfotransferase 1A1. According to some studies, Sult1a1 may have the following features.
Several observations have clear implications for the effectiveness of SULT1A1 as a drug and hormone metabolizing enzyme and its potential role as a disease risk factor. Both SULT1A2 and SULT1A1 share a common set of allele-encoded enzymes that function differently and are associated with individual differences in phenol SULT properties in the liver. Changes in SULT1A1 activity contribute to prostate cancer risk, and the magnitude of the association may vary by ethnicity and by meat consumption.

Target Background

Function

Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the sulfate conjugation of a wide variety of acceptor molecules bearing a hydroxyl or an amine groupe. Sulfonation increases the water solubility of most compounds, and therefore their renal excretion, but it can also result in bioactivation to form active metabolites. Displays broad substrate specificity for small phenolic compounds. Plays an important role in the sulfonation of endogenous molecules such as steroid hormones and 3,3'-diiodothyronin. Mediates the sulfate conjugation of a variety of xenobiotics, including the drugs acetaminophen and minoxidil. Mediates also the metabolic activation of carcinogenic N-hydroxyarylamines leading to highly reactive intermediates capable of forming DNA adducts, potentially resulting in mutagenesis.

Subcellular Location

Cytoplasm.

Protein Families

Sulfotransferase 1 family

Database Links

STRING: 10090.ENSMUSP00000101980

UniGene: Mm.368982

Code	CSB-EP022933MO
Abbreviation	Recombinant Mouse Sult1a1 protein
MSDS	MSDS Datasheet
Size	US$306
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel. Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP022933MO could indicate that this peptide derived from E.coli-expressed Mus musculus (Mouse) Sult1a1. Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP022933MO could indicate that this peptide derived from E.coli-expressed Mus musculus (Mouse) Sult1a1.
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Recombinant Mouse Sulfotransferase 1A1 (Sult1a1)

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