Recombinant Mouse T-cell surface glycoprotein CD8 beta chain(Cd8b),partial

Code CSB-YP004967MO1
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Source Yeast
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Code CSB-EP004967MO1
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Source E.coli
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Code CSB-EP004967MO1-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP004967MO1
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Source Baculovirus
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Code CSB-MP004967MO1
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names Cd8b
Uniprot No. P10300
Alternative Names Cd8b; Cd8b1; Ly-3; Lyt-3; Lyt3; T-cell surface glycoprotein CD8 beta chain; Lymphocyte antigen 3; T-cell membrane glycoprotein Ly-3; T-cell surface glycoprotein Lyt-3; CD antigen CD8b
Species Mus musculus (Mouse)
Protein Length Partial
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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Target Background

Function
(From Uniprot)
Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class I molecule:peptide complex. The antigens presented by class I peptides are derived from cytosolic proteins while class II derived from extracellular proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class I proteins presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. A palmitoylation site in the cytoplasmic tail of CD8B chain contributes to partitioning of CD8 into the plasma membrane lipid rafts where signaling proteins are enriched. Once LCK recruited, it initiates different intracellular signaling pathways by phosphorylating various substrates ultimately leading to lymphokine production, motility, adhesion and activation of cytotoxic T-lymphocytes (CTLs). Additionally, plays a critical role in thymic selection of CD8+ T-cells.
Gene References into Functions
  1. We propose that ADP-ribosylation of CD8-beta can regulate the coreceptor function of CD8 in the presence of elevated levels of extracellular NAD(+) . PMID: 23575529
  2. sialylation in a discrete segment of the CD8beta stalk by ST3Gal-1 sialyltransferase creates a molecular developmental switch that affects ligand binding PMID: 12459555
  3. The stalk region of CD8 beta is capable of fine-tuning the coreceptor function of CD8 proteins as a coreceptor, possibly due to its distinct protein structure, smaller physical size and the unique glycan adducts associated with this region. PMID: 12847256
  4. The stalk region of CD8beta influences the ability of CD8 to bind class I major histocompatibility complexes and is therefore critical for the correct functioning of CD8alphabeta heterodimers. PMID: 19109142
  5. The crystal structure of CD8alphabeta in complex with H-2Dd antigen indicates that CD8beta is crucial in orienting the CD8alphabeta heterodimer. PMID: 19625641

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Subcellular Location Membrane; Single-pass type I membrane protein.
Database Links

KEGG: mmu:12526

STRING: 10090.ENSMUSP00000070131

UniGene: Mm.333148

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