Recombinant Mouse Transcription activator BRG1(Smarca4) ,partial

Code CSB-YP659697MO
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Source Yeast
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Code CSB-EP659697MO
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Source E.coli
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Code CSB-EP659697MO-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP659697MO
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Source Baculovirus
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Code CSB-MP659697MO
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names Smarca4
Uniprot No. Q3TKT4
Alternative Names Smarca4; Baf190a; Brg1; Snf2b; Snf2l4; Transcription activator BRG1; EC 3.6.4.-; ATP-dependent helicase SMARCA4; BRG1-associated factor 190A; BAF190A; Protein brahma homolog 1; SNF2-beta; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 4
Species Mus musculus (Mouse)
Protein Length Partial
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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Target Data

Function Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Component of SWI/SNF chromatin remodeling complexes that carry out key enzymatic activities, changing chromatin structure by altering DNA-histone contacts within a nucleosome in an ATP-dependent manner. Component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex. At the same time, there is increased recruitment of CREBBP to the promoter by a CREST-dependent mechanism, which leads to transcriptional activation. The CREST-BRG1 complex also binds to the NR2B promoter, and activity-dependent induction of NR2B expression involves a release of HDAC1 and recruitment of CREBBP (By similarity). Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a postmitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to postmitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth. SMARCA4/BAF190A may promote neural stem cell self-renewal/proliferation by enhancing Notch-dependent proliferative signals, while concurrently making the neural stem cell insensitive to SHH-dependent differentiating cues. Acts as a corepressor of ZEB1 to regulate E-cadherin transcription and is required for induction of epithelial-mesenchymal transition (EMT) by ZEB1 (By similarity).
Gene References into Functions
  1. Study using conditional Brg1 knockout mice showed that Brg1 interacted with and was recruited by SREBP1c to the promoters of SREBP target genes and optimized the chromatin structure to facilitate SREBP1c binding. Therefore, a previously unrecognized role for Brg1 in hepatic lipid metabolism by portraying Brg1 as an essential epigenetic co-factor for SREBP1c was identified. PMID: 29857051
  2. Brg1 expression is up-regulated by pro-non-alcoholic steatohepatitis stimuli in vitro and in vivo. Hepatocyte-specific Brg1 deletion alleviates non-alcoholic steatohepatitis in mice. PMID: 29890136
  3. results suggest that miR-144-3p contributes to OGD/R-induced neuronal injury in vitro through negatively regulating Brg1/Nrf2/ARE signaling. PMID: 29457851
  4. Data found that Brg1 played important roles in hair cells (HCs) intrinsic polarity maintenance, anchoring outer hair cells base to the Deiter's cells and scar formation of the auditory epithelium. These results demonstrated a requirement for Brg1 activity in development and suggested a role for Brg1 in the proper cellular structure formation of HCs. PMID: 27255603
  5. HDAC9, in cooperation with BRG1 and MALAT1, mediates a critical epigenetic pathway responsible for vascular smooth muscle cells dysfunction. PMID: 29520069
  6. The results demonstrated that restoration of Brg1 during reperfusion could enhance Nrf2-mediated inducible expression of HO-1 during hepatic ischemia-reperfusion injury to effectively increase antioxidant ability to combat against hepatocytes damage. PMID: 28569786
  7. BAF (BRG1/BRM-Associated Factor) chromatin-remodeling complex may adaptively respond to ethanol exposure to protect against a complete loss of miR-9-2 in fetal neural stem cells. PMID: 28438527
  8. Chromatin accessibility at OCT4-bound sites requires the chromatin remodeller BRG1, which is recruited to these sites by OCT4 to support additional transcription factor binding and expression of the pluripotency-associated transcriptome. PMID: 28287392
  9. Data (including data from studies using knockout mice) suggest that Brg1 is phosphorylated by casein kinase 2 (Ck2; Ck2alpha1 and Ck2alpha-prime are catalytic subunits) in proliferating skeletal myoblasts; Brg1 is catalytic subunit of SWI/SNF chromatin-remodeling enzymes; Ck2-mediated phosphorylation of Brg1 appears to regulate myoblast proliferation. (Brg1 = Brahma-related gene 1 protein) PMID: 28939766
  10. BRG1 is a SOX10 co-activator, required to establish the melanocyte lineage and promote expression of genes important for melanocyte function. PMID: 28431046
  11. n keratinocytes, the promoter-enhancer anchoring regions in the gene-rich transcriptionally active TADs are enriched for the binding of chromatin architectural proteins CTCF, Rad21 and chromatin remodeler Brg1. In contrast to gene-rich TADs, gene-poor TADs show preferential spatial contacts with each other, do not contain active enhancers and show decreased binding of CTCF, Rad21 and Brg1 in keratinocytes PMID: 28863138
  12. Data demonstrate that Brg1 plays an essential role in development and homeostasis, including morphogenesis, stem cell differentiation and cell survival in the duodenum. PMID: 27510977
  13. Point mutations in SMARCA4 (also known as BRG1) mapping to the ATPase domain cause loss of direct binding between BAF and PRC1. PMID: 27941795
  14. BRG1 promotes transcription of endothelial Mrtfa and Mrtfb, which elevates expression of SRF and SRF target genes that establish embryonic capillary integrity. PMID: 28729363
  15. RB is necessary for the recruitment of the BRG1 ATPase to DNA double-strand breaks, which stimulates DNA end resection and homologous recombination PMID: 27940962
  16. Cdx members interact with the SWI-SNF complex and make direct contact with Brg1, a catalytic member of SWI-SNF. Both Cdx2 and Brg1 co-occupy a number of Cdx target genes, and both factors are necessary for transcriptional regulation of such targets. Finally, Cdx2 and Brg1 occupancy occurs coincident with chromatin remodeling at some of these loci. PMID: 28082674
  17. BRG1/BRM and c-MYC have an antagonistic relationship regulating the expression of cardiac conduction genes that maintain contractility, which is reminiscent of their antagonistic roles as a tumor suppressor and oncogene in cancer. PMID: 28232072
  18. Results provide insights into the mechanisms by which Brg1 functions, which is in part via the p53 program, to constrain gene expression and facilitate rapid embryonic growth. PMID: 27185875
  19. Brg1 promotes neurogenic radial glial progenitor cell specification but is dispensable for neuronal differentiation PMID: 27067865
  20. SWI/SNF chromatin remodeler subunits Brg1 and Brm are expressed differentially during drug-induced liver injury and regeneration in a mouse model. PMID: 27097303
  21. these findings described a role for BRG1 and BRM in mitochondrial quality control, by regulating mitochondrial number, mitophagy, and mitochondrial dynamics not previously recognized in the adult cardiomyocyte PMID: 27039070
  22. show that epigenetic chromatin remodeling (via a Brg1 and p53/p21-dependent process) determines the aNSCs and progenitor maintenance and responsiveness of neurogenesis. PMID: 26418130
  23. The stress-induced Brg1-G9a/GLP-Dnmt3 interactions and sequence of repressive chromatin assembly on Myh6 promoter illustrates a molecular mechanism by which the heart epigenetically responds to environmental signals. PMID: 26952936
  24. MITF interacts with BRG1 to promote GATA4 expression in cardiac hypertrophy. PMID: 26388265
  25. SMARCA4 is involved in the TOP1 recruitment to general chromatin, which suppresses transcription-associated genomic instability. PMID: 26842758
  26. the role of SMARCA4 and the two SWI/SNF subunits SMARCD2/BAF60B and DPF2/BAF45D in leukaemia, was investigated. PMID: 26571505
  27. Brg1 may play a critical role in neuronal growth by regulating the NR2B-NR2A switch in the postnatal cortex PMID: 26133793
  28. Brg1 has a role in coordinating multiple processes during retinogenesis and is a tumor suppressor in retinoblastoma PMID: 26628093
  29. The data suggest that BRG1 governs Nanog expression via a dual mechanism involving histone deacetylation and nucleosome remodeling. PMID: 26416882
  30. SmarcA4 is required for synapse development and myocyte enhancer factor 2-mediated synapse remodeling PMID: 26459759
  31. integrates two antagonistic calcium-dependent signalling pathways that control myogenic differentiation PMID: 26081415
  32. dispensable in multiple models of postnatal angiogenesis but required for vascular integrity PMID: 25904594
  33. Endocardial Brg1-deficient mouse embryos develop thickened and disorganized semilunar valve cusps PMID: 26100917
  34. MITF-BRG1 interplay thus plays an essential role in transcription regulation in melanoma. PMID: 25803486
  35. Brg1 functions as a positive regulator for cellular proliferation and survival of primary myoblasts via pax7. PMID: 26036967
  36. abnormalities in Brg1 function result in defects associated with neurodevelopmental disorders and autism PMID: 25929186
  37. Brg1 inhibits the dedifferentiation that precedes neoplastic transformation, thus attenuating tumor initiation PMID: 25792600
  38. Data indicate that Smarca4 protein (Brg1) acts at multiple developmental stages to orchestrate B cell development. PMID: 25985234
  39. Our results highlight potential therapeutic value of targeting Brg1 and serve as a proof of concept that targeting the cells of origin of cancer may be of therapeutic relevance. PMID: 25010414
  40. This study show here that loss of Smarcb1 and Smarca4 leads to severe proliferation deficits of granule neuron precursors and a hypoplastic cerebellum. PMID: 25274825
  41. Genome-wide maps of SMARCA4 binding in multiple embryonic mouse tissues reveale tissue-specific in vivo association of SMARCA4 with both active and repressive distant-acting regulatory sequences. PMID: 24752179
  42. Brg1 is dispensable for the survival and function of the progenitor and differentiated cells in the murine intestinal epithelium, it is essential for the maintenance of the stem cell population in a tissue-specific manner. PMID: 23922304
  43. Data suggest that Brg1 and Brm integrate various proinflammatory cues into cell adhesion molecule transactivation in endothelial injury. PMID: 23963727
  44. Brg1 provides the crucial epigenetic link to hypoxia-induced CAM induction and leukocyte adhesion that engenders endothelial malfunction and pathogenesis of hypoxic pulmonary hypertension. PMID: 24042015
  45. Elevated levels of BRG is associated with leukemia. PMID: 24478402
  46. Brg1 is a determinant of context-dependent Kras-driven pancreatic tumorigenesis. PMID: 24561622
  47. A SMARCA2-containing residual SWI/SNF complex underlies the oncogenic activity of SMARCA4 mutant cancers. PMID: 24421395
  48. in epidermal stem cells, p63 regulates the expression of Brg1, which binds to distinct domains within the epidermal differentiation complex and is required for relocation of the epidermal differentiation complex towards the nuclear interior. PMID: 24346698
  49. SWI/SNF enzymes cooperate with SOX10 to directly activate genes that encode components of peripheral myelin. PMID: 23874858
  50. Brahma-related gene 1 bridges epigenetic regulation of proinflammatory cytokine production to steatohepatitis in mice. PMID: 23281043

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Subcellular Location Nucleus
Protein Families SNF2/RAD54 helicase family
Database Links

KEGG: mmu:20586

STRING: 10090.ENSMUSP00000096547

UniGene: Mm.286593

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