Product Details

Purity

>85% (SDS-PAGE)

Target Names

Tfeb

Uniprot No.

Q9R210

Alternative Names

Tfeb; Tcfeb; Transcription factor EB

Species

Mus musculus (Mouse)

Expression Region

1-475

Target Protein Sequence

MASRIGLRMQ LMREQAQQEE QRERMQQQAV MHYMQQQQQQ QQQLGGPPTP AINTPVHFQS PPPVPGEVLK VQSYLENPTS YHLQQSQHQK VREYLSETYG NKFAAHVSPA QGSPKPAPAA SPGVRAGHVL STSAGNSAPN SPMAMLHISS NPEKEFDDVI DNIMRLDSVL GYINPEMQMP NTLPLSSSHL NVYSGDPQVT ASMVGVTSSS CPADLTQKRE LTDAESRALA KERQKKDNHN LIERRRRFNI NDRIKELGML IPKANDLDVR WNKGTILKAS VDYIRRMQKD LQKSRELENH SRRLEMTNKQ LWLRIQELEM QARVHGLPTT SPSGVNMAEL AQQVVKQELP SEDGPGEALM LGPEVPEPEQ MPALPPQAPL PSAAQPQSPF HHLDFSHGLS FGGGGDEGPT GYPDTLGTEH GSPFPNLSKK DLDLMLLDDS LLPLASDPLF STMSPEASKA SSRRSSFSME EGDVL

Protein Length

full length protein

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Target Background

Function

Transcription factor that acts as a master regulator of lysosomal biogenesis, autophagy, lysosomal exocytosis, lipid catabolism, energy metabolism and immune response. Specifically recognizes and binds E-box sequences (5'-CANNTG-3'); efficient DNA-binding requires dimerization with itself or with another MiT/TFE family member such as TFE3 or MITF. Involved in the cellular response to amino acid availability by acting downstream of MTOR: in the presence of nutrients, TFEB phosphorylation by MTOR promotes its cytosolic retention and subsequent inactivation. Upon starvation or lysosomal stress, inhibition of MTOR induces TFEB dephosphorylation, resulting in nuclear localization and transcription factor activity. Specifically recognizes and binds the CLEAR-box sequence (5'-GTCACGTGAC-3') present in the regulatory region of many lysosomal genes, leading to activate their expression, thereby playing a central role in expression of lysosomal genes. Regulates lysosomal positioning in response to nutrient deprivation by promoting the expression of PIP4P1. Acts as a positive regulator of autophagy by promoting expression of genes involved in autophagy. In association with TFE3, activates the expression of CD40L in T-cells, thereby playing a role in T-cell-dependent antibody responses in activated CD4(+) T-cells and thymus-dependent humoral immunity. Specifically recognizes the gamma-E3 box, a subset of E-boxes, present in the heavy-chain immunoglobulin enhancer. Plays a role in the signal transduction processes required for normal vascularization of the placenta. Involved in the immune response to infection by the bacteria S.aureus or S.enterica, acting downstream of protein kinase D (PKD), probably by regulating cytokine and chemokine expression.

Gene References into Functions

TMEM106B drives lung cancer metastasis by inducing TFEB-dependent lysosome synthesis and secretion of cathepsins. PMID: 30013069
Data show that during testicular development, transcriptional factor EB (TFEB) expression was rapidly increased in the testes at the period of 7 days post-partum (dpp) to 14 dpp. PMID: 30360758
Enhancing lysosomal biogenesis and autophagic flux by activating the TFEB protects against cadmium-induced neurotoxicity. PMID: 28240313
TFEB and TFE3 collaborate with each other in activated macrophages and microglia to promote efficient autophagy induction, increased lysosomal biogenesis, and transcriptional upregulation of numerous proinflammatory cytokines PMID: 27171064
Fcgamma receptor activation causes nuclear translocation of TFEB, a transcription factor that boosts expression of lysosome genes. Indeed, Fc receptor activation is accompanied by increased expression of specific lysosomal proteins. Remarkably, TFEB silencing represses the Fcgamma-receptor-mediated enhancements in degradation and bacterial killing. PMID: 27397893
These data indicate that TFE3 and TFEB play a cooperative, rather than redundant, role in the control of the adaptive response of whole-body metabolism to environmental cues such as diet and physical exercise. PMID: 28283651
TFEB regulates PER3 expression via glucose-dependent effects on CLOCK/BMAL1 PMID: 27373683
These data reveal a novel mechanism of TFEB regulation by MTORC1 essential for lysosomal biogenesis. PMID: 28055300
These results suggest that TFEB expression in the striatum of HDQ175/Q7 mice stimulates autophagy and lysosome activity. PMID: 27689619
A functional role for TFEB downregulation in impaired autophagy completion with JAK2 knockdown is implied by the coincident downregulation in TFEB-regulated genes and a restoration of autophagic flux and podocyte permselectivity by TFEB overexpression. PMID: 28424277
Tfe3 and Tfeb are required for the induced expression of Ppargamma2 and subsequently for adipogenic genes. PMID: 28483914
our data indicate that PPAR-alpha mediates antimicrobial responses to mycobacterial infection by inducing TFEB and lipid catabolism. PMID: 28275133
Consistent with reduced transcription factor EB (TFEB) activity, accumulation of phosphorylated TFEB in STUB1-deficient cells resulted in reduced autophagy and reduced mitochondrial biogenesis. These studies reveal that the ubiquitin-proteasome pathway participates in regulating autophagy and lysosomal functions by regulating the activity of TFEB. PMID: 28754656
These findings identify TFEB as a critical mediator of the beneficial effects of exercise on metabolism. PMID: 28011087
Consistent with reduced mTOR activity and increased TFEB levels, loss of C9orf72 enhances autophagic flux, suggesting that C9orf72 is a negative regulator of autophagy PMID: 27875531
these results uncover a potent role for TFEB-mediated autophagy in the pathogenesis of Cd-induced neurotoxicity, suggesting that control of the autophagic pathway by melatonin might provide an important clue for exploring potential targets for novel therapeutics of Cd-induced neurotoxicity. PMID: 27396692
TFEB mediated autophagy is crucial for protection against lipopolysaccharide induced myocardial injury particularly in aging senescent heart. PMID: 27200146
Acute and chronic ethanol administration affects TFEB localization in the liver and regulates hepatic autophagy. PMID: 26556759
TFEB and TFE3 are novel components of the integrated stress response PMID: 26813791
TFEB-regulated signaling pathway for osteoblast differentiation is involved in ATF4/CHOP-dependent signaling pathway. PMID: 26519689
TFEB participates in the Amyloid-beta-induced pathogenesis of Alzheimer's disease by regulating the autophagy-lysosome pathway in transgenic mice model of Alzheimer's disease. PMID: 26368054
Data indicate that transcription factor TFEB is a key participant in the differential regulation of the presentation of exogenous antigens by dendritic cells (DCs). PMID: 26030023
TFEB specifically targets hyperphosphorylated and misfolded Tau species present in both soluble and aggregated fractions while leaving normal Tau intact. PMID: 25069841
We examined the transcriptional regulation of autophagy and observed a functionally significant physical interaction between TFEB and AR in spinal and bulbar muscular atrophy. PMID: 25108912
TFEB was rapidly activated in murine macrophages upon S. aureus infection and was required for proper transcriptional induction of several proinflammatory cytokines and chemokines. PMID: 24882217
Overexpression of TFEB in a new muscle cell culture system and in mouse models of the disease reduced glycogen load and lysosomal size, improved autophagosome processing, and alleviated excessive accumulation of autophagic vacuoles. PMID: 23606558
findings show that TFEB, a master regulator of lysosomal biogenesis and autophagy, is induced by starvation through an autoregulatory feedback loop and exerts a global transcriptional control on lipid catabolism via Ppargc1alpha and Ppar1alpha PMID: 23604321
TFEB alone was capable of reducing htt aggregation and neurotoxicity, placing PGC-1alpha upstream of TFEB. PMID: 22786682
TFEB is identified as a master regulator of lysosomal biogenesis and function. PMID: 19556463
TFEB phosphorylation and nuclear localization is regulated coordinately by mTORC1 in a manner dependent on both TFEB and V-ATPases. Phosphorylation of the C-terminal serine motif is necessary and sufficient for nuclear translocation. PMID: 21804531
study found that starvation activates a transcriptional program controlling major steps of the autophagic pathway; TFEB, a master gene for lysosomal biogenesis, coordinated this program by driving expression of autophagy and lysosomal genes PMID: 21617040
TFE3 and TFEB regulate E-cadherin and WT1 expression PMID: 15994295
Maximum Cd40lg promoter activity and gene expression required TFE3 or TFEB. PMID: 16936731
first report describing the increased expression of TFEB in bladder inflammation in response to protease activated receptor (PAR) activation; findings suggest that this molecule may influence the participation of mast cells in PAR-mediated inflammation PMID: 17705868

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Subcellular Location

Cytoplasm, cytosol. Lysosome membrane. Nucleus.

Protein Families

MiT/TFE family

Tissue Specificity

Widely expressed.

Database Links

KEGG: mmu:21425

STRING: 10090.ENSMUSP00000024786

UniGene: Mm.2305

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Recombinant Mouse Transcription factor EB (Tfeb)

Product Details

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