Recombinant Mouse Tryptophan 5-hydroxylase 1 (Tph1)

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Code CSB-EP024100MO
MSDS
Size US$306
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Product Details

Purity
Greater than 90% as determined by SDS-PAGE.
Target Names
Tph1
Uniprot No.
Research Area
Signal Transduction
Alternative Names
Tph1; Tph; Tryptophan 5-hydroxylase 1; EC 1.14.16.4; Tryptophan 5-monooxygenase 1
Species
Mus musculus (Mouse)
Source
E.coli
Expression Region
1-447aa
Target Protein Sequence
MIEDNKENKENKDHSSERGRVTLIFSLENEVGGLIKVLKIFQENHVSLLHIESRKSKQRNSEFEIFVDCDISREQLNDIFPLLKSHATVLSVDSPDQLTAKEDVMETVPWFPKKISDLDFCANRVLLYGSELDADHPGFKDNVYRRRRKYFAELAMNYKHGDPIPKIEFTEEEIKTWGTIFRELNKLYPTHACREYLRNLPLLSKYCGYREDNIPQLEDVSNFLKERTGFSIRPVAGYLSPRDFLSGLAFRVFHCTQYVRHSSDPLYTPEPDTCHELLGHVPLLAEPSFAQFSQEIGLASLGASEETVQKLATCYFFTVEFGLCKQDGQLRVFGAGLLSSISELKHALSGHAKVKPFDPKIACKQECLITSFQDVYFVSESFEDAKEKMREFAKTVKRPFGLKYNPYTQSVQVLRDTKSITSAMNELRYDLDVISDALARVTRWPSV
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight
67.3kDa
Protein Length
Full Length
Tag Info
N-terminal 6xHis-SUMO-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Oxidizes L-tryptophan to 5-hydroxy-l-tryptophan in the rate-determining step of serotonin biosynthesis.
Gene References into Functions
  1. Both gut-specific Tph1 knockout mice and liver-specific Htr2a knockout mice are resistant to high fat diet-induced hepatic steatosis, without affecting systemic energy homeostasis. PMID: 30446669
  2. Data suggest that central melanocortin-4 receptor (MC4-R) and tryptophan hydroxylase (TPH) are involved in the efferent neuronal control of the kidneys. PMID: 27626491
  3. Prolactin-induced Tph1 expression. PMID: 26136513
  4. Tph1-deficient mice fed a high-fat diet (HFD) are protected from obesity, insulin resistance and nonalcoholic fatty liver disease (NAFLD) while exhibiting greater energy expenditure by brown adipose tissue. PMID: 25485911
  5. Lack of tryptophan hydroxylase-1 in mice results in gait abnormalities. PMID: 23516593
  6. ZBP-89 directly binds to the Tph1 promoter, which is required for its basal and butyrate-inducible expression. PMID: 23395646
  7. TPH1 deficiency or inhibition reduces allergic airway inflammation. Platelet-derived 5-HT is pivotal in AAI and lack of 5-HT leads to impaired Th2 priming capacity of bone marrow dendritic cells. PMID: 23328530
  8. Data indicate that tryptophan hydroxylase (Tph)1-deficient mice, lacking non-neuronal serotonin, showed mild leukocytosis. PMID: 23243271
  9. Data show that Tph-1 (tryptophan hydroxylase-1) is a potent regulator of immunity. PMID: 23008335
  10. Parallel to Tph1 mRNA and protein induction, islet serotonin content increased to a peak level that was 200-fold higher than basal. Interestingly, only a subpopulation of the beta cells was serotonin-positive in vitro and in vivo. PMID: 20938637
  11. nonneuronal tph1 gene has a role in cardiac function with peripheral serotonin PMID: 14597720
  12. Review. TPH1 is expressed in the pineal gland and the enterochromaffin cells. Loss of TPH1 gene expression, and thus of peripheral 5-HT, leads to a cardiac dysfunction phenotype. PMID: 15146950
  13. estrogen receptor-beta is responsible for mediating estrogen regulated tryptophan hydroxylase-1 expression in the murine dorsal raphe nucleus PMID: 15820717
  14. TPH1 is expressed preferentially during late developmental brain stages. Low raphe nuclei 5-HT contents were seen during development in NZW & SWR mice with common functional polymorphisms in TPH1. TPH1 may influence behavior later in life. PMID: 16407550
  15. TPH1 is the predominant form expressed in pineal gland and with a molecular weight of 51 kDa. PMID: 16581041
  16. Tryptophan hydroxylase 1 and peripheral serotonin play an essential role in the development of hypoxia-induced elevations in pulmonary pressures and hypoxia-induced pulmonary vascular remodeling. PMID: 17130306
  17. Tph1 knockout mice used to show that serotonin plays a role in the pathogenesis of steatohepatitis. PMID: 17681180
  18. Cardiomyopathy is more severe in adult Tph1 knockout mice born to homozygous mothers than to heterozygous mothers. PMID: 18267982
  19. In a genetic model of serotonin deficiency (Tph1(-/-)) in mice, serotonin shown to be crucial for the growth of s.c. colon cancer allografts in vivo. PMID: 18593914
  20. substantially reduced peripheral 5-HT levels in Tph1/Tph2 double knockout, but not TPH2KO mice PMID: 18923670
  21. In mice no significant TPH1 expression was detected in the brain. PMID: 19181488

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Protein Families
Biopterin-dependent aromatic amino acid hydroxylase family
Database Links
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