Recombinant Mouse V-set domain containing T-cell activation inhibitor 1(Vtcn1)

Code CSB-YP742524MO
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Source Yeast
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Code CSB-EP742524MO
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Source E.coli
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Code CSB-EP742524MO-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP742524MO
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Source Baculovirus
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Code CSB-MP742524MO
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names Vtcn1
Uniprot No. Q7TSP5
Alternative Names Vtcn1; B7h4; V-set domain containing T-cell activation inhibitor 1; B7 homolog 4; B7-H4; Immune costimulatory protein B7-H4; Protein B7S1; T cell costimulatory molecule B7x
Species Mus musculus (Mouse)
Expression Region 25-257
Target Protein Sequence LIIGFG ISGKHFITVT TFTSAGNIGE DGTLSCTFEP DIKLNGIVIQ WLKEGIKGLV HEFKEGKDDL SQQHEMFRGR TAVFADQVVV GNASLRLKNV QLTDAGTYTC YIRTSKGKGN ANLEYKTGAF SMPEINVDYN ASSESLRCEA PRWFPQPTVA WASQVDQGAN FSEVSNTSFE LNSENVTMKV VSVLYNVTIN NTYSCMIEND IAKATGDIKV TDSEVKRRSQ LQLLNSG
Protein Length Full Length of Mature Protein
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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Target Background

Function
Negatively regulates T-cell-mediated immune response by inhibiting T-cell activation, proliferation, cytokine production and development of cytotoxicity. When expressed on the cell surface of tumor macrophages, plays an important role, together with regulatory T-cells (Treg), in the suppression of tumor-associated antigen-specific T-cell immunity. Involved in promoting epithelial cell transformation.
Gene References into Functions
  1. this study shows that B7-H4 knockout hosts mount greater tumor-associated anti-tumor T cell responses and display reduced tumors PMID: 28074226
  2. Results highlight an importance of VTCN1 stabilization on cell surfaces for the restoration of altered balance of immune control during T1D. PMID: 26773144
  3. B7-H4 expression by nonhematopoietic cells in the tumor microenvironment promotes antitumor immunity. PMID: 25527357
  4. that B7x can modulate kidney damage in autoimmune diseases including lupus nephritis and anti-glomerular basement membrane disease PMID: 25205493
  5. B7-H4 can inhibit both antitumor T cells and protumor myeloid-derived suppressor cells ( PMID: 23686485
  6. The endogenous expression of B7-H4 in donor beta cells from transgenic mice prolongs islet allograft survival, confirming the negative role of B7-H4 in regulating alloreactive T-cell responses. PMID: 23192157
  7. Local overexpression of B7x on pancreatic beta cells is sufficient to abolish CD8 T cell-induced diabetes. PMID: 22972920
  8. An enhanced T cell response contributes to reduced lethality in a pulmonary infection model with Streptococcus pneumoniae. PMID: 22855708
  9. B7-H4 expression may influence the outcome of T-cell tumor cell interactions. Tumor-associated macrophage induced membrane-bound B7-H4 on lung cancer cell represents a novel mechanism by which lung cancer cells evade immune recognition and destruction. PMID: 22108530
  10. Overexpression of B7-H4 was shown in tumor infiltrated dendritic cells. PMID: 21728826
  11. Tissue-specific expression of B7x protects from CD4 T cell-mediated autoimmunity. PMID: 21727190
  12. intact recipient, but not donor, B7-H4 is essential for prolongation of allograft survival by blocking CD28/CTLA4:B7 pathway using CTLA4-Ig. PMID: 21143433
  13. studies reveal a novel role for activated hepatic stellate cells in the attenuation of intrahepatic T cell responses by way of expression of the coinhibitory molecule B7-H4. PMID: 21064155
  14. May participate in negative regulation of cell-mediated immunity in peripheral tissues PMID: 12818165
  15. novel negative costimulator and regulates the threshold of T cell activation PMID: 12818166
  16. Genomic DNA of mouse B7-H4 is mapped on chromosome 3 comprised of six exons and five introns spanning 66 kb. PMID: 14568939
  17. These results suggest that B7-H4 is one of multiple negative cosignaling molecules that collectively provide a fine-tuning mechanism for T-cell-mediated immune responses. PMID: 16914726
  18. T-cell and epithelial cell crosstalk-associated B7-H4 induces T-cell activation and is a potential activator that promotes tubular lesion PMID: 17051145
  19. B7-H4 is a negative regulator of the neutrophil response to infection PMID: 19109567
  20. Local expression of B7-H4 prolongs islet allograft survival in vivo, suggesting translational potential for beta-cell replacement with reduced immune injury. PMID: 19307783
  21. these results suggest a different action of B7-H4 in T cell-mediated inflammatory responses and the possible involvement of soluble B7-H4 in inflammatory immune responses. PMID: 19723502

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Subcellular Location Cell membrane; Lipid-anchor, GPI-anchor.
Protein Families Immunoglobulin superfamily, BTN/MOG family
Tissue Specificity Expressed on the surface of professional antigen-presenting cells (at protein level). Widely expressed, including in kidney, liver, lung, pancreas, placenta, prostate, spleen, testis and thymus.
Database Links

KEGG: mmu:242122

STRING: 10090.ENSMUSP00000057721

UniGene: Mm.137467

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