Recombinant Mouse mammary tumor virus Gag polyprotein (gag)

Code CSB-YP319171MIT
MSDS
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Source Yeast
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Code CSB-EP319171MIT
MSDS
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Source E.coli
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Code CSB-EP319171MIT-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP319171MIT
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP319171MIT
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
gag
Uniprot No.
Alternative Names
gagGag polyprotein [Cleaved into: Matrix protein p10; Phosphorylated protein pp21; Protein p3; Protein p8; Protein n; Capsid protein p27; Nucleocapsid protein p14]
Species
Mouse mammary tumor virus (strain BR6) (MMTV)
Expression Region
2-99
Target Protein Sequence
GVSGSKGQK LFVSVLQRLL SERGLHVKES SAIEFYQFLI KVSPWFPEEG GLNLQDWKRV GREMKRYAAE HGTDSIPKQA YPIWLQLREI LTEQSDLVL
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Matrix protein.; Binds strongly to viral nucleic acids and promotes their aggregation. Also destabilizes the nucleic acids duplexes via highly structured zinc-binding motifs.; Capsid protein.
Gene References into Functions
  1. The crystal structure of N-myristoylated matrix protein, determined at 1.57 A resolution, revealed that the myristoyl groups are buried in a hydrophobic pocket at the dimer interface and contribute to dimer formation. PMID: 26728401
  2. MMTV Gag targets the pericentriolar region. PMID: 26121257
  3. These data support the hypothesis that efficient mouse mammary tumor virus particle assembly is dependent upon the interaction of Gag and L9 in the nucleoli of infected cells. PMID: 23135726
  4. Expression of mouse mammary tumor virus Gag requires nuclear interactions involving the viral Rem protein, its cognate binding target the RmRE, and surprisingly, both a splice donor and acceptor sequence. PMID: 22277305
Subcellular Location
[Matrix protein p10]: Virion.; [Capsid protein p27]: Virion.; [Nucleocapsid protein p14]: Virion.
Database Links

KEGG: vg:1491864

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