Product Details

Purity

Greater than 85% as determined by SDS-PAGE.

Target Names

Vwf

Uniprot No.

Q8CIZ8

Research Area

Cardiovascular

Alternative Names

Vwfvon Willebrand factor; vWF) [Cleaved into: von Willebrand antigen 2; von Willebrand antigen II)]

Species

Mus musculus (Mouse)

Source

E.coli

Expression Region

1498-1665aa

Target Protein Sequence

DVVFVLEGSDEVGEANFNKSKEFVEEVIQRMDVSPDATRISVLQYSYTVTMEYAFNGAQSKEEVLRHVREIRYQGGNRTNTGQALQYLSEHSFSPSQGDRVEAPNLVYMVTGNPASDEIKRLPGDIQVVPIGVGPHANMQELERISRPIAPIFIRDFETLPREAPDLV
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

23.9 kDa

Protein Length

Partial

Tag Info

N-terminal 10xHis-tagged and C-terminal Myc-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

The recombinant Mouse Vwf protein is encoded by the gene of Vwf (1498-1665aa). The gene of Vwf was cloned in a system (E.coli) that supported the expression of Vwf and translation of messenger RNA. Modification of Vwf by recombinant DNA technology could lead to the expression of the target protein. The protein was fused with N-terminal 10xHis tag & C-terminal Myc tag in the production. The purity is 85% determined by SDS-PAGE.

Vwf is a protein coding gene that encodes von Willebrand factor. According to some studies, Vwf may have the following features.
Assay of vWF-cleaving proteases based on decreased collagen-binding affinity of degraded vWF. Type 1 VWD is characterized by a partial quantitative deficiency of vWF. The full-length vWF cDNA encodes a highly repetitive protein that is much larger than the mature vWF subunit. The platelet-VWF complex is the preferred substrate for ADAMTS13 under fluid shear stress. Inhibition of the VWF-collagen interaction with an anti-human VWF monoclonal antibody resulted in abolished arterial platelet thrombus formation in baboons. The phenotype of VWF survival was found in a subgroup of patients with type 1 VWD. Low VWF levels may be associated with massive bleeding, mainly due to reduced VWF synthesis and/or constitutive secretion.

Target Background

Function

Important in the maintenance of hemostasis, it promotes adhesion of platelets to the sites of vascular injury by forming a molecular bridge between sub-endothelial collagen matrix and platelet-surface receptor complex GPIb-IX-V. Also acts as a chaperone for coagulation factor VIII, delivering it to the site of injury, stabilizing its heterodimeric structure and protecting it from premature clearance from plasma.

Gene References into Functions

is synthesized in megakaryocytes and endothelial cells (ECs) and has two main roles: to carry and protect coagulation factor VIII (FVIII) from degradation by forming VWF-FVIII complex; and to mediate platelet adhesion and aggregation at sites of vascular injury. PMID: 29392565
this study shows that Von Willebrand factor protects against acute CCl4-induced hepatotoxicity through phospho-p38 MAPK signaling pathway inhibition PMID: 28868583
the ADAMTS13-vWF axis is partially involved in the pathophysiology of kidney ischemic reperfusion injury. PMID: 27507004
Type 2N von Willebrand disease variants were associated with decreased VWF secretion and impaired factor VIII binding/stability. PMID: 28581694
identify a critical role for VWF in cerebral inflammation and blood-brain barrier damage after intracerebral haemorrhage PMID: 27782211
Refrigeration-induced binding of VWF to platelets facilitates their rapid clearance by inducing GPIbalpha-mediated signaling. PMID: 29097365
BLOC-2 subunit HPS6 deficiency affects the tubulation and secretion of von Willebrand factor from mouse endothelial cells PMID: 27889498
A novel single-domain antibody against von Willebrand factor A1 domain that interferes with VWF-platelet interactions in vivo. By using this sdAb, show that the A1 domain is pertinent to the participation of VWF in the inflammatory response. PMID: 28642239
These experiments delineate an unexpected pathway in which microbiota-triggered TLR2 signaling alters the synthesis of proadhesive VWF by the liver endothelium and favors platelet integrin-dependent thrombus growth. PMID: 28572286
ADAMTS13 controls vascular remodeling by modifying VWF reactivity during stroke recovery. PMID: 28428179
these novel findings support the hypothesis that conformation of the VWF A domains plays a critical role in modulating macrophage-mediated clearance of VWF in vivo. PMID: 27554083
results revealed localized vascular expression of FVIII and von Willebrand factor and identified lymphatic endothelial cell as a major cellular source of FVIII in extrahepatic tissues. PMID: 27207787
Endothelial cell derivedVWF is the major determinant that mediates VWF-dependent ischemic stroke by promoting postischemic thrombo-inflammation. PMID: 27444201
VWF deficiency reduces the progression of liver fibrosis, suggesting a mechanistic role of elevated plasma VWF levels in cirrhosis PMID: 28527913
von Willebrand factor exerts beneficial effects in a mouse sepsis model via recruitment of neutrophils to inflammatory sites. PMID: 26494840
Staphylococcus lugdunensis binds directly to von Willebrand factor, which proved to be vital for withstanding shear forces and for its adhesion to the vessel wall and cardiac valves. PMID: 26743845
Clinical experimental cerebral malaria progression was delayed, and overall survival was significantly prolonged in VWF(-/-) mice compared with WT controls. PMID: 26511133
in stable compensated heart failure mice, disruptions in endothelial vWF expression and extrusion may reduce the incidence of endocardial thrombosis PMID: 26565707
VWF is expressed in a mosaic pattern in the capillaries of many vascular beds and in the aorta. Hearts of VWF-null mice demonstrate an abnormal endothelial phenotype as well as cardiac dysfunction. PMID: 26744078
SNAP23 Regulates Endothelial Exocytosis of von Willebrand Factor PMID: 26266817
Both platelet-VWF and plasma-VWF are required for optimal platelet-derived FVIII gene therapy for hemophilia A in the presence of inhibitors. PMID: 25955153
a genetic link between EGLN1 and VWF in a constitution specific manner which could modulate thrombosis/bleeding susceptibility and outcomes of hypoxia, is reported. PMID: 26047609
novel findings demonstrate a specific and critical role for the R1205 residue in modulating macrophage-mediated clearance of VWF in vivo PMID: 25690668
Clearance differences between blood group O and non-blood group O individuals may therefore be related to the blood group status of the individual rather than the ABH antigen loading on VWF itself. PMID: 25650553
Certain VWD-type 2B mutations relieve the need for shear stress to induce LRP1 binding. Enhanced LRP1 binding coincides with a reduced survival of VWF/p.R1306Q and VWF/p.V1316M PMID: 25728415
These data suggest that whereas platelet-derived VWF does not play a crucial role in hemostasis and arterial thrombosis, it aggravates thrombo-inflammatory diseases such as stroke via a GPIb-dependent mechanism. PMID: 26209660
Platelet-endothelial interactions occur in early atherosclerosis. These interactions are in part caused by endothelial von Willebrand factor large multimers, which can be reversed with exogenous ADAMTS13. PMID: 26156014
Data suggest that targeting platelet receptor glycoprotein Ibalpha (GPIbalpha)-von Willebrand factor VWF-A1 binding interface may offer a therapeutic approach to reducing platelet-driven thrombosis. PMID: 25293780
Absence of vWF increases hematopoiesis in long-term bone marrow cultures and has a protective effect in irradiated lungs. PMID: 24982209
VWF-binding protein contributes to vascular adhesion of S aureus through 2 independent mechanisms: shear-mediated binding to VWF and activation of prothrombin to form S aureus-fibrin-platelet aggregates. PMID: 24951431
a delayed and markedly reduced thrombogenic response was still evident in VWF(-/-), GPVI, and alpha2beta1 blocked animals, suggesting that alternative primary hemostatic mechanisms can partially rescue the bleeding phenotype associated with these defects. PMID: 25051961
a fragment containing only approximately 20% of the VWF sequence is sufficient to support FVIII stability in vivo PMID: 24850761
Expression of VWF/p.S1494C-p.A1534C in mice triggers an acute onset of thrombotic thrombocytopenic purpura. PMID: 24713928
Following endothelial damage, platelet cross-linking during closure of the vessel lumen is mediated by GPIbalpha-VWF interactions. PMID: 24553181
The results indicate that elevation of extracellular sodium within the physiological range raises vWF sufficiently to increase coagulability and risk of thrombosis. PMID: 24733925
Galpha12 may play an important role in promoting membrane trafficking and exocytosis for basal and thrombin-induced vWF secretion, in a process involving SNAP, Galpha12/13 and Galphaq/11 PMID: 24081657
Carboxyl terminus of ADAMTS13 directly inhibits platelet aggregation and ultra large von Willebrand factor string formation under flow in a free-thiol-dependent manner. PMID: 24357063
VWF represents a promising target for the treatment of cutaneous inflammation, e.g., leukocytoclastic vasculitis. PMID: 23812299
VWF has a role in leukocyte recruitment with chromatin decondensation by PAD4, which increases myocardial ischemia/reperfusion injury in mice PMID: 24200682
Platelet aggregation, secretion, and spreading were diminished due to inhibition of integrin alphaIIbbeta3 in platelets from mice expressing a vWD-type 2B-associated vWF (vWF/p.V1316M). PMID: 24270421
a new function for VWF in vivo as regulator of bloodstream thrombopoiesis PMID: 23737952
VWF type 2B binds to platelets, which is a signal for clearance by macrophages, possibly contributing to thrombocytopenia PMID: 23945153
VWF deficiency confers partial preservation of blood brain barrier integrity after hypoxia/reoxygenation and seizures. PMID: 23825365
Compared with wild-type mice (n=6), we found less bacteria in postcapillary (60+/-6 versus 32+/-5 bacteria) and collecting venules (48+/-5 versus 18+/-4 bacteria; P<0.05) of VWF knockout mice (n=5). PMID: 23720451
Hypoxia is associated with a phenotypic shift which target regulation of VWD in lung endothelial cells. PMID: 23580145
These findings implicate a role for intronic splicing in mediating lineage-specific expression of vWF in the endothelium. PMID: 23529929
ADAMTS13 and VWF are causally involved in myocardial ischemia/reperfusion injury. PMID: 22983446
Provide new evidence for ADAMTS13 in reducing VWF-mediated acute cerebral inflammation following ischemic stroke. PMID: 22712744
Endothelial cell (EC) VWF is sufficient to support hemostasis in VWF-/- mice, and VWF produced in megakaryocytes/platelets can also contribute to hemostasis in the absence of EC-derived VWF. PMID: 22642380
study showed O-glycosylations are dispensable for normal VWF multimerization and biosynthesis; it appears that some O-glycosylation sites, particularly the T1255 and T1256 residues, are involved in maintenance of VWF plasma levels and are essential for normal haemostasis PMID: 22616016

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Subcellular Location

Secreted. Secreted, extracellular space, extracellular matrix.

Tissue Specificity

Plasma. Expressed in liver.

Database Links

KEGG: mmu:22371

STRING: 10090.ENSMUSP00000001995

UniGene: Mm.22339

Code	CSB-EP025960MO
Abbreviation	Recombinant Mouse Vwf protein, partial
MSDS	MSDS Datasheet
Size	US$306
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Recombinant Mouse von Willebrand factor (Vwf), partial

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