Product Details

Purity

>85% (SDS-PAGE)

Target Names

sir-2.1

Uniprot No.

Q21921

Alternative Names

sir-2.1; R11A8.4; NAD-dependent protein deacetylase sir-2.1; EC 2.3.1.286; Protein sir-2.1; Regulatory protein SIR2 homolog 1

Species

Caenorhabditis elegans

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Target Background

Function

NAD-dependent deacetylase. Required for a reduction of the 'Lys-16' acetylation of histone H4 (H4K16ac) on dosage-compensated X chromosomes in hermaphrodites. Plays a role in germ cell and somatic cell apoptosis in response to DNA damage. Functions upstream of daf-16 in the insulin-like signaling pathway, promoting daf-16 mediated transcriptional activation and increased lifespan. May also regulate lifespan independently of daf-16 by modulating the transcription of genes involved in the stress response of the endoplasmic reticulum (ER). Acts upstream of the nicotinic acid metabolism pathway, which may be linked to the regulation of longevity. Plays a role in ascaroside-mediated longevity and stress resistance.

Gene References into Functions

SIRT1 plays essential roles in cell survival, inflammation, energy metabolism, and aging. PMID: 25976661
The authors show that the mating circuits' functional durability depends on the metabolic regulator SIR-2.1, a NAD(+)-dependent histone deacetylase. PMID: 24755287
Sir-2.1; dnj-14 double mutants showed full lifespan rescue by resveratrol. PMID: 24947438
C. elegans produces specific small molecules to control adult lifespan in a SIR-2.1-dependent manner. PMID: 23509272
Overexpression of SIR2.1 protects neurons from mutant huntingtin toxicity. PMID: 22956852
caloric restriction increases the ability of heat shock to preserve movement in a polyglutamine toxicity neurodegenerative disease model and that this effect is dependent on sir2.1. PMID: 22778258
calorie restriction affords, an sir-2.1 mediated, protection against the dopaminergic neurodegeneration, that might have implications for neurodegenerative Parkinson's disease. PMID: 21889494
data suggest that SIRT1 activators have therapeutic potential in prion diseases to protect neurons from the detrimental effects of misfolded PrP conformers on intracellular signaling and homeostasis PMID: 20392961
activation of Sirtuin-1 (by the pharmacological agent resveratrol and/or genetic means) per se ignites autophagy, and that Sirtuin-1 is required for the autophagic response to nutrient deprivation PMID: 20023410
SIR-2.1 and 14-3-3 act in parallel to the insulin-like pathway to activate DAF-16 and extend life span. PMID: 16777605
Dietary restriction (DR) can robustly extend the lifespan of worms lacking the AAK2 or SIR-2.1, identifying universal regulators of DR mediated longevity PMID: 19229346
Data indicate that SIR-2.1 and HIS-24 contribute to the propagation of a specialized chromatin state at the subtelomeric regions and elsewhere in the genome. PMID: 19380489

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Subcellular Location

Nucleus. Cytoplasm.

Protein Families

Sirtuin family, Class I subfamily

Database Links

KEGG: cel:CELE_R11A8.4

STRING: 6239.R11A8.4a

UniGene: Cel.12479

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Recombinant NAD-dependent protein deacetylase sir-2.1 (sir-2.1), partial

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