Recombinant Nuclear hormone receptor family member nhr-67 (nhr-67)

Code CSB-YP895486CXY
MSDS
Size Pls inquire
Source Yeast
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Code CSB-EP895486CXY
MSDS
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Source E.coli
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Code CSB-EP895486CXY-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP895486CXY
MSDS
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Source Baculovirus
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Code CSB-MP895486CXY
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
nhr-67
Uniprot No.
Alternative Names
nhr-67; C08F8.8; Nuclear hormone receptor family member nhr-67
Species
Caenorhabditis elegans
Expression Region
1-416
Target Protein Sequence
MMTAVSQMSV PSSRILLDVD CRVCEDHSSG KHYSIFSCDG CAGFFKRSIR RHRQYVCKNK GSPSEGQCKV DKTHRNQCRA CRLRKCLEIG MNKDAVQHER GPRNSSLRRQ QMMFDHGSSP NSPEMGSESD AIILPTSSMN RDTVAGTAAR IFFALVGFCQ NPLNGVPKER QMTMFQQNWA ALLVLHATET RAITSKQIRT ETISGSSEQR NAVANAFEII ERLQLDNREY MMLKHFTMWR DTPSAIQIVF QLASIQNFTH RTEPTRYIQC INAIAAIPTT SIIDVLFRPS IGSASMPRLI QDMFKPPQQP TPTSLFPMAN FNLNFLLKQE KTETEEGEDI EEEDDATSSN QFDENSSTDD RSVGELDPVQ LFLALNSSTQ PSSASSPSSS RPRHSIRSIT ELLSIQEEES VNVEEV
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Orphan nuclear receptor that binds DNA containing an extended core motif half-site sequence 5'-AAGTCA-3'. In males, plays an essential role in the migration of the linker cell which guides gonad elongation during the L3 and L4 stages of larval development by negatively regulating the expression of netrin receptor unc-5 at the mid-L3 stage. Involved in the regulation of non-apoptotic cell death in the linker cell, acting upstream of or in parallel to transcription factor hsf-1.
Gene References into Functions
  1. nhr-67 functions in the development of the Caenorhabditis elegans ventral uterus. PMID: 21718694
  2. nhr-67 regulates patterning of gene expression and morphogenesis in the C. elegans vulva. PMID: 17465684
  3. that the difference between FAX-1 and NHR-67 binding preference is partially mediated by a conserved subclass-specific asparagine or aspartate residue at position 19 of the DNA-binding domain PMID: 18179707
  4. Data show that the C. elegans Tailless/TLX-type, orphan nuclear receptor NHR-67 acts at several distinct steps to determine the identity and subsequent left/right (L/R) asymmetric subtype diversification of a class of gustatory neurons, the ASE neurons. PMID: 19641012
  5. nhr-67 regulates a stage-specific program of linker cell migration in male gonadogenesis. PMID: 19906858

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Subcellular Location
Nucleus.
Protein Families
Nuclear hormone receptor family
Tissue Specificity
Expressed in linker cell.
Database Links

KEGG: cel:CELE_C08F8.8

STRING: 6239.C08F8.8

UniGene: Cel.12545

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