Recombinant Oryza sativa subsp. japonica Heme oxygenase 1, chloroplastic (HO1)

Code CSB-YP731673OFG
MSDS
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Source Yeast
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Code CSB-EP731673OFG
MSDS
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Source E.coli
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Code CSB-EP731673OFG-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP731673OFG
MSDS
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Source Baculovirus
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Code CSB-MP731673OFG
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
HO1
Uniprot No.
Alternative Names
HO1; HY1; SE5; Os06g0603000; LOC_Os06g40080; OsJ_21897; P0486H12.31; Heme oxygenase 1; chloroplastic; EC 1.14.14.18; Protein PHOTOPERIODIC SENSITIVITY 5
Species
Oryza sativa subsp. japonica (Rice)
Expression Region
65-289
Target Protein Sequence
AAATAA EMAPAASGEE GKPFVEEMRA VAMRLHTKDQ AKEGEKEPQA PPVARWEPSV DGYLRFLVDS KLVFETLETI VDRAAVPWYA EFRNTGLERS EQLKKDLEWF KEQGHTIPEP SAPGTTYASY LEELAEKDSQ AFICHFYNVY FAHTAGGRMI GKKVSENILN KKELEFYKWE GNLSQLLQNV RNKLNEVASS WTREEKDHCL DETEKSFSYS GDLLRHIFT
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Catalyzes the opening of the heme ring to form the open-chain tetrapyrrole biliverdin IX with the release of iron and carbon monoxide (CO). Is a key enzyme in the synthesis of the chromophore of the phytochrome family of plant photoreceptors. Essential for photoperiod response and repression of flowering through cytochromes that inhibit flowering by affecting both HD1 and EHD1 flowering pathways.
Gene References into Functions
  1. the activity of l-DES, as well as the enzymes involved in nitrogen metabolism, was significantly increased in the OsSE5-overexpression line. PMID: 28474399
  2. Results suggest that SE5 (GenBank accession no. EU781632) may be useful for molecular breeding designed to improve plant tolerance to oxidative stress. PMID: 22829206
Subcellular Location
Plastid, chloroplast.
Protein Families
Heme oxygenase family
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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