Product Details

Purity

>85% (SDS-PAGE)

Target Names

par-6

Uniprot No.

Q9NAN2

Alternative Names

par-6; T26E3.3Partitioning defective protein 6

Species

Caenorhabditis elegans

Expression Region

1-309

Target Protein Sequence

MSYNGSYHQN HHSTLQVKSK FDSEWRRFSI PMHSASGVSY DGFRSLVEKL HHLESVQFTL CYNSTGGDLL PITNDDNLRK SFESARPLLR LLIQRRGESW EEKYGYGTDS DKRWKGISSL MAQKPPKRSY SISNPEDFRQ VSAIIDVDIV PEAHRRVRLC KHGQERPLGF YIRDGTSVRV TERGVVKVSG IFISRLVDGG LAESTGLLGV NDEVLEVNGI EVLGKTLDQV TDMMVANAHN LIITVKPANQ RNTLSRGPSQ QGTPNASEMS AATAAATGGI QRPMKMNGSS DGSYHPKQHD ANDSDSGED

Protein Length

full length protein

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Target Background

Function

Necessary for apicobasal and anterior-posterior asymmetries associated with cell adhesion and gastrulation during the first few cell cycles of embryogenesis. Required for localizing/ maintaining par-3 at the cell periphery. Regulates mes-1 expression and/or localization pattern during early embryogenesis. Acts together with par-3 and pkc-3 in maintaining epithelial cell polarity in the distal spermatheca. Plays a role in endosome and Golgi body positioning.

Gene References into Functions

we find that vab-1 lies in the same genetic pathway as cdc-42 and is responsible for polarizing CDC-42 activity to the medial tip. Together, these data establish a previously uncharacterized role for polarized CDC-42, in conjunction with PAR-6, PAR-3 and an Eph receptor(vab-1), during epithelial intercalation. PMID: 27861585
the arcade cell epithelium polarizes by a PAR-6-mediated pathway that is independent of E-cadherin, beta-integrin and beta-laminin. PMID: 25773364
PAR-3 and PAR-6 function in cytokinesis may be partially redundant and play a role in the maintenance of DYN-1 in the cleavage furrow. PMID: 22887994
evidence PAR polarity arises from coupling of advective transport by flowing cell cortex to a multistable PAR reaction-diffusion system; advection in active, flowing medium provides mechanism for mechanically templated pattern formation in development PMID: 22021673
Centrosomes localize apically by first moving toward lateral foci of the conserved polarity proteins PAR-3 and PAR-6, then move together with these foci toward the future apical surface. Embryos lacking PAR-3 fail to localize their centrosomes apically. PMID: 22425160
PAR-2 and PAR-6, which localize to opposing PAR domains, undergo exchange between well mixed cytoplasmic populations and laterally diffusing membrane-associated states PMID: 21518794
Binding to PKC-3, but not to PAR-3 or to a conventional PDZ domain ligand, is required for PAR-6 function in C. elegans PMID: 20122916
PAR proteins function in both apicobasal and anterior-posterior asymmetry during the first few cell cycles of embryogenesis. PMID: 13129846
CDC-37-mediated inhibition of the CDC-42-dependent binding site and PAR-3-mediated release of this inhibition provide a key mechanism for the anterior accumulation of PAR-6. PMID: 16943281
CDC-42 interaction with PAR-6 is not required for the initial establishment of asymmetry but is required for maximal cortical accumulation of PAR-6 and to maintain its asymmetry. PMID: 16996049
PAR-6 is required for junction formation but not apicobasal polarization in C. elegans embryonic epithelial cells. PMID: 17314130
nos-3 loss of function mutant suppresses the lethality of par-2 mutants by regulating PAR-6 protein levels. PMID: 18502413
study identified a RhoGAP, PAC-1, which mediates embryo radial polarity & gastrulation by excluding PAR-6 from contacted cell surfaces; PAC-1 provides a dynamic molecular link between cell contacts & PAR proteins that polarizes embryos radially PMID: 18583611

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Subcellular Location

Cytoplasm. Cell membrane; Peripheral membrane protein. Cell junction, tight junction.

Protein Families

PAR6 family

Tissue Specificity

Colocalized with par-3 at all stages in early embryos, at the anterior cortex of the embryo. Patchy expression observed at the periphery after completion of meiosis I and in meiosis II, which on completion of metaphase II, is restricted to the anterior 85

Database Links

KEGG: cel:CELE_T26E3.3

STRING: 6239.T26E3.3a.3

UniGene: Cel.8562

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Recombinant Partitioning defective protein 6 (par-6)

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