Recombinant Phaeosphaeria nodorum Autophagy-related protein 3(ATG3)

Code CSB-YP604325EUG
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Source Yeast
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Code CSB-EP604325EUG
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Source E.coli
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Code CSB-EP604325EUG-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP604325EUG
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Source Baculovirus
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Code CSB-MP604325EUG
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names ATG3
Uniprot No. Q0U388
Alternative Names ATG3; SNOG_13776; Autophagy-related protein 3; Autophagy-related E2-like conjugation enzyme ATG3
Species Phaeosphaeria nodorum (strain SN15 / ATCC MYA-4574 / FGSC 10173) (Glume blotch fungus) (Parastagonospora nodorum)
Expression Region 1-350
Target Protein Sequence MAYNFVRSYI DTFRERTAAI SHTSTFRETG QITPEEFVLA GDFLVFKFPS WQWGDASSAG KRVPYLPEGK QYLMTRGVPC HRRLDDNFAG EAGQDETIVG DGFAGSEGAD DDGWLRTGGM AASQEAKVRD VRTVDESGNL GAAEEEDEIP DMEDMDDDEE AIIRDPQGGS SSTAPLRTYT LYICYSAHYR TPRLYLSGYG ATSVPLKPQE MMEDIVGDYK DKTVTIEDFP FFEHALKTAS VHPCKHASVM KVLLDRADAA LKLRLAKLKA GKDIGKLDTG MEGLVDDTRL LKLSEQAKGK EGDEAKDDWE VLSEGGDDEV AIRVDQYLVV FLKFMASVTP GIEHDFTMGV
Protein Length full length protein
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

Target Data

Function E2 conjugating enzyme required for the cytoplasm to vacuole transport (Cvt) and autophagy. Required for selective autophagic degradation of the nucleus (nucleophagy) as well as for mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Responsible for the E2-like covalent binding of phosphatidylethanolamine to the C-terminal Gly of ATG8. The ATG12-ATG5 conjugate plays a role of an E3 and promotes the transfer of ATG8 from ATG3 to phosphatidylethanolamine (PE). This step is required for the membrane association of ATG8. The formation of the ATG8-phosphatidylethanolamine conjugate is essential for autophagy and for the cytoplasm to vacuole transport (Cvt). The ATG8-PE conjugate mediates tethering between adjacent membranes and stimulates membrane hemifusion, leading to expansion of the autophagosomal membrane during autophagy (By similarity).
Subcellular Location Cytoplasm
Protein Families ATG3 family
Database Links

KEGG: pno:SNOG_13776

STRING: 13684.SNOT_13776

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