Recombinant Pig 2'-5'-oligoadenylate synthase 1 (OAS1)

Code CSB-YP640913PI
MSDS
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Source Yeast
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Code CSB-EP640913PI
MSDS
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Source E.coli
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Code CSB-EP640913PI-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP640913PI
MSDS
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Source Baculovirus
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Code CSB-MP640913PI
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
OAS1
Uniprot No.
Alternative Names
OAS1; 2'-5'-oligoadenylate synthase 1; (2-5')oligo(A) synthase 1; 2-5A synthase 1; EC 2.7.7.84; p42 OAS
Species
Sus scrofa (Pig)
Expression Region
1-349
Target Protein Sequence
MELRHTPARD LDKFIEDHLL PNTCFRTQVK EAIDIVCRFL KERCFQGTAD PVRVSKVVKG GSSGKGTTLR GRSDADLVVF LTKLTSFEDQ LRRRGEFIQE IRRQLEACQR EQKFKVTFEV QSPRRENPRA LSFVLSSPQL QQEVEFDVLP AFDALGQWTP GYKPNPEIYV QLIKECKSRG KEGEFSTCFT ELQRDFLRNR PTKLKSLIRL VKHWYQTCKK THGNKLPPQY ALELLTVYAW EQGSRKTDFS TAQGFQTVLE LVLKHQKLCI FWEAYYDFTN PVVGRCMLQQ LKKPRPVILD PADPTGNVGG GDTHSWQRLA QEARVWLGYP CCKNLDGSLV GAWTMLQKI
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Interferon-induced, dsRNA-activated antiviral enzyme which plays a critical role in cellular innate antiviral response. In addition, it may also play a role in other cellular processes such as apoptosis, cell growth, differentiation and gene regulation. Synthesizes higher oligomers of 2'-5'-oligoadenylates (2-5A) from ATP which then bind to the inactive monomeric form of ribonuclease L (RNase L) leading to its dimerization and subsequent activation. Activation of RNase L leads to degradation of cellular as well as viral RNA, resulting in the inhibition of protein synthesis, thus terminating viral replication. Can mediate the antiviral effect via the classical RNase L-dependent pathway or an alternative antiviral pathway independent of RNase L. The secreted form displays antiviral effect against vesicular stomatitis virus (VSV), herpes simplex virus type 2 (HSV-2), and encephalomyocarditis virus (EMCV) and stimulates the alternative antiviral pathway independent of RNase L.
Gene References into Functions
  1. Comparison of day 25 Meishan and white composite swine placentas by microarray expression profiling revealed a breed-specific transition expression polymorphism in OAS1. PMID: 18510738
  2. The active sites of OAS and CCA class I enzymes are highly conserved. PMID: 18604630
Subcellular Location
Cytoplasm. Mitochondrion. Nucleus. Microsome. Endoplasmic reticulum. Secreted.
Protein Families
2-5A synthase family
Database Links

UniGene: Ssc.1031

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