Recombinant Plasmodium falciparum Gametocyte surface protein P230 (PFS230), partial

Code CSB-YP302412EWP
MSDS
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Source Yeast
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Code CSB-EP302412EWP-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP302412EWP
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP302412EWP
MSDS
Size Pls inquire
Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
PFS230
Uniprot No.
Alternative Names
PFS230; PF230; S230Gametocyte surface protein P230
Species
Plasmodium falciparum (isolate 3D7)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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 Q&A
Q:

I want to know if it was possible to biotinylated this protein. Do you guys offer biotin labeling?

A:
Thanks for your inquiry.
If you request the Biotin label, we can provide the biotinylated service, It's labeled in vitro by random.
It's labeled at the free amino terminal of all lysines (primaquine) not only at the N-terminus, which means the amino acids containing the lysine residues can all be labelled.
There will be extra charges and the lead time will be extended for 3 working days.

Target Background

Function
Gametocyte surface protein required for male/female gamete fusion. Also required for male gamete exflagellation and interaction with erythrocytes.
Gene References into Functions
  1. the identification, biochemical, biophysical, and immunological characterization of recombinant Pfs230 domains. PMID: 27432885
  2. Data show that anti-Pfs230 and Pf48/45 antibodies developed rapidly after exposure to gametocytes and were strongly associated with transmission-reducing activity. PMID: 21124765
  3. These results suggest that Pfs230 is the surface molecule on males that mediates RBC binding and plays an important role in oocyst development, a critical step in malaria transmission. PMID: 16879650
Subcellular Location
Cell surface. Cell membrane.
Database Links

KEGG: pfa:PFB0405w

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