Recombinant Probable endonuclease III homolog (nth-1)

Code CSB-YP347471CXY
MSDS
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Source Yeast
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Code CSB-EP347471CXY
MSDS
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Source E.coli
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Code CSB-EP347471CXY-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP347471CXY
MSDS
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Source Baculovirus
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Code CSB-MP347471CXY
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
nth-1
Uniprot No.
Alternative Names
nth-1; R10E4.5; Endonuclease III homolog; CeNTH; EC 3.2.2.-; EC 4.2.99.18; Bifunctional DNA N-glycosylase/DNA-(apurinic or apyrimidinic site) lyase; DNA glycosylase/AP lyase
Species
Caenorhabditis elegans
Expression Region
1-298
Target Protein Sequence
MHSFSMKRVV ASSSVAAVAT CDVEGTVVAW RRDVELIRKM RKDMIAPVDT MGCHKLADPL AAPPVHRFQV LVALMLSSQT RDEVNAAAMK RLKDHGLSIG KILEFKVPDL ETILCPVGFY KRKAVYLQKT AKILKDDFSG DIPDSLDGLC ALPGVGPKMA NLVMQIAWGE CVGIAVDTHV HRISNRLGWI KTSTPEKTQK ALEILLPKSE WQPINHLLVG FGQMQCQPVR PKCGTCLCRF TCPSSTAKNV KSETEETSTS IEVKQEVEDE FEDEKPAKKI KKTRKTRTKI EVKTESET
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Bifunctional DNA N-glycosylase with associated apurinic/apyrimidinic (AP) lyase function that catalyzes the first step in base excision repair (BER), the primary repair pathway for the repair of oxidative DNA damage. The DNA N-glycosylase activity releases the damaged DNA base from DNA by cleaving the N-glycosidic bond, leaving an AP site. The AP lyase activity cleaves the phosphodiester bond 3' to the AP site by a beta-elimination. Primarily recognizes and repairs oxidative base damage of pyrimidines.
Gene References into Functions
  1. we could detect no deficiency in base excision repair in the nth-1 strain. PMID: 22959841
  2. Data show that the protein encoded by the sequence containing both R10E4.5 and the 117-bp region upstream from it, and found that the protein had the NTH activity. PMID: 19481506
Subcellular Location
Nucleus. Mitochondrion.
Protein Families
Nth/MutY family
Database Links

KEGG: cel:CELE_R10E4.5

STRING: 6239.R10E4.5d

UniGene: Cel.10201

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