Recombinant Probable syndecan (sdn-1), partial

Code CSB-YP341895CXY
MSDS
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Source Yeast
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Code CSB-EP341895CXY
MSDS
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Source E.coli
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Code CSB-EP341895CXY-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP341895CXY
MSDS
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Source Baculovirus
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Code CSB-MP341895CXY
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
sdn-1
Uniprot No.
Alternative Names
sdn-1; F57C7.3; Probable syndecan
Species
Caenorhabditis elegans
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Cell surface proteoglycan that bears heparan sulfate. Required for correct mitotic spindle orientation of the ABar blastomere division plane and this may be through modulation of astral microtubule array, and in association with the wnt-signaling proteins mig-5 and dsh-2. Involved in the migration of AQR and PQR neurons, which descend from the Q neuroblasts. Promotes the axon guidance of D-type motor neurons.
Gene References into Functions
  1. SDN-1 controls cell migration in nervous system in coordination with Wnt signaling. PMID: 28576860
  2. The sdn-1 mutants showed QR descendant AQR anterior migration defects, and weaker QL descendant PQR migration defects. PMID: 26022293
  3. Syndecan's core protein, not its sugar chains, mediates growth cone stabilization. Syndecan also functions in axon guidance via its sugar chains. PMID: 25001284
  4. SDN-1 is required for precise orientation of the mitotic spindle in response to a Wnt cue in development. PMID: 25344071
  5. syndecan (SDN-1) is required for normal egg laying and associates with the nervous system and the vulva PMID: 15456854
  6. distinct heparan sulfate modification patterns on SDN-1 are involved in regulating axon guidance and cell migration in C. elegans PMID: 16176946
  7. SDN-1 affects UNC-5 function by regulating the proper extracellular distribution of growth factors PMID: 19631636

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Subcellular Location
Membrane; Single-pass type I membrane protein. Cell surface. Cell junction. Cytoplasm.
Protein Families
Syndecan proteoglycan family
Database Links

STRING: 6239.F57C7.3a.1

UniGene: Cel.23080

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