Recombinant Protein fantom (mks-5), partial

Code CSB-YP600629CXY
MSDS
Size Pls inquire
Source Yeast
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Code CSB-EP600629CXY
MSDS
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Source E.coli
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Code CSB-EP600629CXY-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP600629CXY
MSDS
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Source Baculovirus
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Code CSB-MP600629CXY
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
mks-5
Uniprot No.
Alternative Names
mks-5; ftm; nphp-8; C09G5.8Protein fantom; Meckel syndrome protein homolog
Species
Caenorhabditis elegans
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Thought to have an important role in cilia formation and cilia-mediated chemosensation. Involved in the docking of other MKS/MKSR proteins localized to the transition zone of the cilia.
Gene References into Functions
  1. findings expand the repertoire of MKS module-associated proteins and suggest an MKS-5 and CEP-290-dependent assembly pathway for building a functional ciliary transition zone PMID: 26982032
  2. MKS-5 acts to exclude membrane-associated signalling proteins from the transition zone and limits PIP2 ciliary abundance, creating an optimal, discrete ciliary signalling compartment. PMID: 26392567
  3. these data suggest that NPHP-8/RPGRIP1L plays an important role in cilia formation and cilia-mediated chemosensation in a cell type-specific manner. PMID: 21689635
Subcellular Location
Cell projection, cilium. Note=Localizes to the transition zone.
Protein Families
RPGRIP1 family
Tissue Specificity
Expressed at the transition zone at the base of cilia. Expressed in ciliated sensory neurons, including the amphid neurons in the head.
Database Links

KEGG: cel:CELE_C09G5.8

STRING: 6239.C09G5.8a

UniGene: Cel.15908

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