Recombinant Pseudomonas aeruginosa B-type flagellin (fliC)

Code CSB-YP300968EZX
MSDS
Size Pls inquire
Source Yeast
Have Questions? Leave a Message or Start an on-line Chat
Code CSB-EP300968EZX
MSDS
Size Pls inquire
Source E.coli
Have Questions? Leave a Message or Start an on-line Chat
Code CSB-EP300968EZX-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
Have Questions? Leave a Message or Start an on-line Chat
Code CSB-BP300968EZX
MSDS
Size Pls inquire
Source Baculovirus
Have Questions? Leave a Message or Start an on-line Chat
Code CSB-MP300968EZX
MSDS
Size Pls inquire
Source Mammalian cell
Have Questions? Leave a Message or Start an on-line Chat

Product Details

Purity
>85% (SDS-PAGE)
Target Names
fliC
Uniprot No.
Alternative Names
fliC; PA1092B-type flagellin
Species
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Expression Region
1-488
Target Protein Sequence
MALTVNTNIA SLNTQRNLNA SSNDLNTSLQ RLTTGYRINS AKDDAAGLQI SNRLSNQISG LNVATRNAND GISLAQTAEG ALQQSTNILQ RIRDLALQSA NGSNSDADRA ALQKEVAAQQ AELTRISDTT TFGGRKLLDG SFGTTSFQVG SNAYETIDIS LQNASASAIG SYQVGSNGAG TVASVAGTAT ASGIASGTVN LVGGGQVKNI AIAAGDSAKA IAEKMDGAIP NLSARARTVF TADVSGVTGG SLNFDVTVGS NTVSLAGVTS TQDLADQLNS NSSKLGITAS INDKGVLTIT SATGENVKFG AQTGTATAGQ VAVKVQGSDG KFEAAAKNVV AAGTAATTTI VTGYVQLNSP TAYSVSGTGT QASQVFGNAS AAQKSSVASV DISTADGAQN AIAVVDNALA AIDAQRADLG AVQNRFKNTI DNLTNISENA TNARSRIKDT DFAAETAALS KNQVLQQAGT AILAQANQLP QAVLSLLR
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

 Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Target Background

Function
Flagellin is the subunit protein which polymerizes to form the filaments of bacterial flagella.
Gene References into Functions
  1. Study indicates the roles of FliC phosphorylation in the processes of motility, secretion and biofilm formation and uncovered effects of S/T phosphorylation of FliC on protease secretion by T2SS and biofilm dispersal. PMID: 27701473
  2. Passive immunization with antibodies against flagellin a+b led to an efficacious protection against P. aeruginosa infection in both acute pneumonia and burn models. PMID: 28065582
  3. Flagellin drives NEU1 to desialylate MUC1, thereby increasing its adhesiveness for Pseudomonas aeruginosa and its shedding PMID: 25963144
  4. Administration of flagellin 'b' via intraperitoneal route enhanced the clearance of homologous as well as heterologous bacteria (P. aeruginosa uroisolate carrying flagellin 'a') in renal tissue PMID: 25455605
  5. Data suggest that the structure-based modeling study on paFliC, the paFliC-TLR5 complex, and the paFliC filament could contribute to the improvement of vaccine design to control P. aeruginosa infection. PMID: 24434155
  6. Flagellum-deficient (DeltafliC) bacteria produces adequate amounts of exoproteases to degrade SP-A through quorum-sensing. PMID: 21205009
  7. The pattern of gene expression directed by flagellin in human airway epithelial cells includes innate host defense genes, proinflammatory cytokines, and chemokines. PMID: 15494517
  8. Flagellin exclusively inhibits the ENaC sodium channel, probably due to release of ATP and activation of purinergic receptors of the P2Y subtype. This activates the MAPK pathway, inhibiting ENaC. Thus, P. aeruginosa reduces Na+ absorption. PMID: 16410345

Show More

Hide All

Subcellular Location
Secreted. Bacterial flagellum.
Protein Families
Bacterial flagellin family
Database Links

KEGG: pae:PA1092

STRING: 208964.PA1092

icon of phone
Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
icon of address
Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
icon of social media
Join us with

Subscribe newsletter

Leave a message

* To protect against spam, please pass the CAPTCHA test below.
CAPTCHA verification
© 2007-2024 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1