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Recombinant Pyrococcus furiosus DNA double-strand break repair Rad50 ATPase (rad50), partial

Recombinant Pyrococcus furiosus DNA double-strand break repair Rad50 ATPase (rad50), partial

Code	CSB-YP348867FHW
MSDS	MSDS
Size	Pls inquire
Source	Yeast
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Code	CSB-EP348867FHW
MSDS	MSDS
Size	Pls inquire
Source	E.coli
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Code	CSB-EP348867FHW-B
MSDS	MSDS
Size	Pls inquire
Source	E.coli
Conjugate	Avi-tag Biotinylated E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code	CSB-BP348867FHW
MSDS	MSDS
Size	Pls inquire
Source	Baculovirus
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Code	CSB-MP348867FHW
MSDS	MSDS
Size	Pls inquire
Source	Mammalian cell
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Product Details
Customer Reviews and Q&A
Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

rad50

Uniprot No.

P58301

Alternative Names

rad50; PF1167; DNA double-strand break repair Rad50 ATPase; pfRad50

Species

Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Customer Reviews and Q&A

■ Customer Reviews

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Target Background

Function

Part of the Rad50/Mre11 complex, which is involved in the early steps of DNA double-strand break (DSB) repair. The complex may facilitate opening of the processed DNA ends to aid in the recruitment of HerA and NurA. Rad50 controls the balance between DNA end bridging and DNA resection via ATP-dependent structural rearrangements of the Rad50/Mre11 complex. The ATP-bound conformation promotes DNA end binding and end tethering, and alters Mre11 nuclease activity. ATP hydrolysis promotes both Mre11 activity as well as HerA/NurA activity. Has also reversible adenylate kinase activity.

Gene References into Functions

Together, these data suggest an equilibrium between a ground state and an "active" dimerization competent state of Rad50 that has locally altered structure and dynamics and is poised for ATP-induced dimerization and eventual ATP hydrolysis. PMID: 29374232
Study shows that at limiting levels of HerA and NurA, PfMre11/Rad50 is required for 5' end resection and that this process is dependent upon the enzymatic activities of HerA, NurA, and Rad50 but not Mre11. PMID: 18957200

Protein Families

SMC family, RAD50 subfamily

Database Links

KEGG: pfu:PF1167

STRING: 186497.PF1167

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