Recombinant Ras-like GTP-binding protein rhoA (rho-1)

Code CSB-YP638718CXY
MSDS
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Source Yeast
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Code CSB-EP638718CXY
MSDS
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Source E.coli
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Code CSB-EP638718CXY-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP638718CXY
MSDS
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Source Baculovirus
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Code CSB-MP638718CXY
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
rho-1
Uniprot No.
Alternative Names
rho-1; rhoa; Y51H4A.3; Ras-like GTP-binding protein rhoA
Species
Caenorhabditis elegans
Expression Region
1-189
Target Protein Sequence
MAAIRKKLVI VGDGACGKTC LLIVFSKDQF PDVYVPTVFE NYVADIEVDG KQVELALWDT AGQEDYDRLR PLSYPDTDVI LMCFSIDSPD SLENIPEKWT PEVRHFCPNV PIILVGNKRD LRSDPQTVRE LAKMKQEPVK PEQGRAIAEQ IGAFAYLECS AKTKDGIREV FEKATQAALQ QKKKKKSKC
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Required for ventral migration of epidermal cells during ventral enclosure in the embryo and for cell elongation. Also required for ventral migration of P cells during larval development. Involved in asymmetric spindle positioning during anaphase and establishment of cell polarity during embryo development. In adults, involved in regulation of multiple processes including locomotion, pharyngeal pumping, fecundity, ovulation, defecation and body morphology. In body wall muscles, regulates organization of myosin thick filaments downstream of unc-89. Association with the oxidase bli-3 promotes ROS production and this interaction may be modulated by memo-1, in order to control the oxidative stress response and longevity.
Gene References into Functions
  1. Here, the authors report that in the Caenorhabditis elegans zygote, feedback between active RhoA and myosin induces a contractile instability in the actomyosin cell cortex. PMID: 28117665
  2. RHO-1 RhoGTPase modulates fertility and multiple behaviors in adult C. elegans. PMID: 21387015
  3. Presynaptic changes in Rho activity at Caenorhabditis elegans neuromuscular junctions can radically change animal behavior via modulation of two separate pathways affecting a neurotransmitter release PMID: 16391233
  4. RHO-1 activity is required to facilitate the segregation of CDC-42 and PAR-6 to the anterior. PMID: 16899536
  5. The upstream GPA-12/RHGF-1 pathway stimulates only a subset of RHO-1 downstream effectors, suggesting that either the RHO-1 effectors require different levels of activated RHO-1 or there are two distinct pools of RHO-1 within C. elegans neurons. PMID: 17139250

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Subcellular Location
Cell membrane; Lipid-anchor; Cytoplasmic side. Cytoplasm. Cytoplasm, cytoskeleton. Cytoplasm, cell cortex.
Protein Families
Small GTPase superfamily, Rho family
Tissue Specificity
In larvae and adults, enriched at the tip of the head where the anterior sensory organ is located and in the pharyngeal nerve ring (at protein level). In embryos, enriched at the boundaries of dorsal cells undergoing intercalation, ventral enclosure and e
Database Links

KEGG: cel:CELE_Y51H4A.3

STRING: 6239.Y51H4A.3

UniGene: Cel.17449

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