Recombinant Rat ADP-ribosylation factor GTPase-activating protein 1 (Arfgap1)

Code CSB-YP726814RA
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Source Yeast
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Code CSB-EP726814RA
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Source E.coli
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Code CSB-EP726814RA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP726814RA
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Source Baculovirus
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Code CSB-MP726814RA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Arfgap1
Uniprot No.
Alternative Names
Arfgap1; Arf1gapADP-ribosylation factor GTPase-activating protein 1; ARF GAP 1; ADP-ribosylation factor 1 GTPase-activating protein; ARF1 GAP; ARF1-directed GTPase-activating protein
Species
Rattus norvegicus (Rat)
Expression Region
1-415
Target Protein Sequence
MASPRTRKVL KEVRAQDENN VCFECGAFNP QWVSVTYGIW ICLECSGRHR GLGVHLSFVR SVTMDKWKDI ELEKMKAGGN AKFREFLEAQ DDYEPSWSLQ DKYSSRAAAL FRDKVATLAE GKEWSLESSP AQNWTPPQPK TLQFTAHRPA GQPQNVTTSG DKAFEDWLND DLGSYQGAQE NRYVGFGNTV PPQKREDDFL NSAMSSLYSG WSSFTTGASK FASAAKEGAT KFGSQASQKA SELGHSLNEN VLKPAQEKVK EGRIFDDVSS GVSQLASKVQ GVGSKGWRDV TTFFSGKAED TSDRPLEGHS YQNSSGDNSQ NSTIDQSFWE TFGSAEPPKA KSPSSDSWTC ADASTGRRSS DSWDIWGSGS ASNNKNSNSD GWESWEGASG EGRAKATKKA APSTAADEGW DNQNW
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
GTPase-activating protein (GAP) for the ADP ribosylation factor 1 (ARF1). Involved in membrane trafficking and /or vesicle transport. Promotes hydrolysis of the ARF1-bound GTP and thus, is required for the dissociation of coat proteins from Golgi-derived membranes and vesicles, a prerequisite for vesicle's fusion with target compartment. Probably regulates ARF1-mediated transport via its interaction with the KDELR proteins and TMED2. Overexpression induces the redistribution of the entire Golgi complex to the endoplasmic reticulum, as when ARF1 is deactivated. Its activity is stimulated by phosphoinosides and inhibited by phosphatidylcholine.
Gene References into Functions
  1. these findings point to mechanistic differences between ArfGAP1 and the other ArfGAPs known to function in the COPI system. PMID: 20211604
  2. Data show that casein kinase I is required for protein traffic through the early secretory pathway and can modulate the amount of ADP-ribosylation factor-GTPase activating protein (ARF-GAP1) that can bind to membranes. PMID: 12181329
  3. ALPS1 is the primary determinant of the interaction of ArfGAP1 with lipid membranes and that ALPS2 reinforces this interaction 40-fold; ArfGAP1 responds to a wide range of membrane curvature and adapts to lipid membranes of various acyl chain compositions PMID: 17253781
  4. Differential roles of ArfGAP1, ArfGAP2, and ArfGAP3 in COPI trafficking PMID: 19015319
Subcellular Location
Cytoplasm. Golgi apparatus. Note=Associates with the Golgi complex.
Tissue Specificity
Widely expressed. Highly expressed in brain and liver.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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