Recombinant Rat Alpha-adducin (Add1)

Code CSB-YP733906RA
MSDS
Size Pls inquire
Source Yeast
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Code CSB-EP733906RA
MSDS
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Source E.coli
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Code CSB-EP733906RA-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP733906RA
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP733906RA
MSDS
Size Pls inquire
Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Add1
Uniprot No.
Alternative Names
Add1Alpha-adducin; Erythrocyte adducin subunit alpha
Species
Rattus norvegicus (Rat)
Expression Region
1-735
Target Protein Sequence
MNGDTRAAVV TSPPPTTAPH KERYFDRVDE NNPEYLRERN MAPDLRQDFN MMEQKKRVSM ILQSPAFCEE LESMIQEQFK KGKNPTGLLA LQQIADFMTA SVPNVYPAAP QGGMAALNMS LGMVTPVNDL RGSDSIAYDK GEKLLRCKLA AFYRLADLFG WSQLIYNHIT TRVNSEQEHF LIVPFGLLYS EVTASSLVKV NLQGDIVDRG STNLGVNQAG FTLHSAIYAA RPDAKCIVHI HTPAGAAVSA MKCGLLPISP EALSLGEVAY HDYHGILVDE EEKILIQKNL GPKSKVLILR NHGLVSVGES VEEAFYYIHN LVVACEIQVR TLASAGGPDN LVLLDPGKYK AKSRSPGTPA GEGSGSPPKW QIGEQEFEAL MRMLDNLGYR TGYPYRYPAL RERSKKYSDV EVPASVTGHS FASDGDSGTC SPLRHSFQKQ QREKTRWLNS GRGDDASEEG QNGSSPKSKT KWTKEDGHRT STSAVPNLFV PLNTNPKEVQ EMRNKIREQN LQDIKTAGPQ SQVLCGVMMD RSLVQGELVT ASKAIIEKEY QPHVIVSTTG PNPFNTLTDR ELEEYRREVE RKQKGSEENL DETREQKEKS PPDQSAVPNT PPSTPVKLEG GLPQEPTSRD GSDATTSKPT PPDLSPDEPS EALAFPTVAE EAHASPDTTQ PLAEADPEPA SASAPGAEEV ASPATEEGSP MDPGSDGSPG KSPSKKKKKF RTPSFLKKSK KKSDS
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Membrane-cytoskeleton-associated protein that promotes the assembly of the spectrin-actin network. Binds to calmodulin.
Gene References into Functions
  1. Altered expression of renal aquaporins and alpha-adducin polymorphisms may contribute to the establishment of salt-sensitive hypertension. PMID: 21451595
  2. Reorganization of the unique cytoskeletal network defining a primary quiescent T cell is mediated thru the TCR-dependent modification & downregulation of alpha-adducin, resulting in a cytoskeletal shape compatible with T cell effector & memory functions. PMID: 15163540
  3. Add1 has a role in regulating blood pressure PMID: 15474463
  4. overexpression of wild type alpha adducin (WT-Add1) in endothelial cells induced capillary-like structure development PMID: 17512505
  5. The constitutive reduction of the Na/K pump endocytic rate induced by mutated adducin variants may be relevant in Na-dependent hypertension. PMID: 18524856

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Subcellular Location
Cytoplasm, cytoskeleton. Cell membrane; Peripheral membrane protein; Cytoplasmic side.
Protein Families
Aldolase class II family, Adducin subfamily
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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