Recombinant Rat Bcl-2-binding component 3 (Bbc3)

Code CSB-YP802262RA
MSDS
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Source Yeast
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Code CSB-EP802262RA
MSDS
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Source E.coli
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Code CSB-EP802262RA-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP802262RA
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP802262RA
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Bbc3
Uniprot No.
Alternative Names
Bbc3; PumaBcl-2-binding component 3; p53 up-regulated modulator of apoptosis
Species
Rattus norvegicus (Rat)
Expression Region
1-193
Target Protein Sequence
MARARQEGSS PEPVEGLARD SPRPFPLGRL MPSAVSCGLC EPGLPAAPAA PALLPAAYLC APTAPPAVTA ALGGPRWPGG HRSRPRGPRP DGPQPSLSPA QQHLESPVPS APEALAGGPT QAAPGVRVEE EEWAREIGAQ LRRMADDLNA QYERRRQEEQ HRHRPSPWRV MYNLFMGLLP LPRDPGAPEM EPN
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Essential mediator of p53/TP53-dependent and p53/TP53-independent apoptosis. Functions by promoting partial unfolding of BCL2L1 and dissociation of BCL2L1 from p53/TP53. Essential for ER stress-induced cell death.
Gene References into Functions
  1. These results demonstrated the involvement of BBC3/Bbc3 in cold preservation/rewarming-mediated islet injury, possibly through modulating HMGB1- and oxidative stress-mediated injury to islets. PMID: 27117005
  2. Reduction of p53 upregulated modulator of apoptosis (PUMA) expression mediates the endogenous cardioprotective mechanisms of postconditioning by disrupting mitochondrial apoptotic pathway. PMID: 26043893
  3. we identified a novel AP1-binding site on rat puma gene which is necessary for direct binding of c-Jun with Puma promoter PMID: 25891762
  4. over-expression of PUMA correlates with cauda equina compression caused apoptosis of spinal cord cells PMID: 23658678
  5. An in vivo model of aorta-caval shunt and aortic banding showed increased PUMA protein expression in the aorta. PMID: 22021910
  6. Data show that transcriptional activation of PUMA is regulated by nuclear factor-kappaB and endoplasmic reticulum stress. PMID: 20421300
  7. Our study thus establishes Puma as an essential mediator of cardiomyocyte death upon I/R injury and offers a novel therapeutic target to limit cell loss in ischemic heart disease. PMID: 16399862
  8. These results imply a potential role for PUMA in delayed neuronal death after transient global cerebral ischemia PMID: 19095966

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Subcellular Location
Mitochondrion.
Protein Families
Bcl-2 family
Database Links
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