Recombinant Rat Carnitine O-palmitoyltransferase 2, mitochondrial (Cpt2)

Code CSB-YP005925RA
MSDS
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Source Yeast
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Code CSB-EP005925RA
MSDS
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Source E.coli
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Code CSB-EP005925RA-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP005925RA
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP005925RA
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Cpt2
Uniprot No.
Alternative Names
Cpt2; Cpt-2Carnitine O-palmitoyltransferase 2; mitochondrial; EC 2.3.1.21; Carnitine palmitoyltransferase II; CPT II
Species
Rattus norvegicus (Rat)
Expression Region
26-658
Target Protein Sequence
SAVSG PDDYLQHSIV PTMHYQDSLP RLPIPKLEDT MKRYLNAQKP LLDDSQFRRT EALCKNFETG VGKELHAHLL AQDKQNKHTS YISGPWFDMY LTARDSIVLN FNPFMAFNPD PKSEYNDQLT RATNLTVSAV RFLKTLQAGL LEPEVFHLNP SKSDTDAFKR LIRFVPPSLS WYGAYLVNAY PLDMSQYFRL FNSTRIPRPN RDELFTDTKA RHLLVLRKGH FYVFDVLDQD GNIVNPLEIQ AHLKYILSDS SPVPEFPVAY LTSENRDVWA ELRQKLIFDG NEETLKKVDS AVFCLCLDDF PMKDLIHLSH TMLHGDGTNR WFDKSFNLIV AEDGTAAVHF EHSWGDGVAV LRFFNEVFRD STQTPAITPQ SQPAATNSSA SVETLSFNLS GALKAGITAA KEKFDTTVKT LSIDSIQFQR GGKEFLKKKQ LSPDAVAQLA FQMAFLRQYG QTVATYESCS TAAFKHGRTE TIRPASIFTK RCSEAFVRDP SKHSVGELQH MMAECSKYHG QLTKEAAMGQ GFDRHLYALR YLATARGLNL PELYLDPAYQ QMNHNILSTS TLNSPAVSLG GFAPVVPDGF GIAYAVHDDW IGCNVSSYSG RNAREFLHCV QKCLEDIFDA LEGKAIKT
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Involved in the intramitochondrial synthesis of acylcarnitines from accumulated acyl-CoA metabolites. Reconverts acylcarnitines back into the respective acyl-CoA esters that can then undergo beta-oxidation, an essential step for the mitochondrial uptake of long-chain fatty acids and their subsequent beta-oxidation in the mitochondrion. Active with medium (C8-C12) and long-chain (C14-C18) acyl-CoA esters.
Gene References into Functions
  1. Low CPT-II expression might lead to abnormal hepatic lipid accumulation, which should promote the malignant transformation of hepatocytes. PMID: 28127199
  2. Report interaction between carnitine palmitoyltransferase 2 and carnitine/acylcarnitine translocase supporting the channeling of acylcarnitines and carnitine at level of the inner mitochondrial membrane. PMID: 24898781
  3. role of highly conserved C-terminal acidic residues glutamate 487 and glutamate 500 on catalytic activity in liver PMID: 12200419
  4. crystal structure of carnitine palmitoyltransferase 2 PMID: 16615913
  5. surface patch mediates the association of CPT-II with the inner membrane of the mitochondria PMID: 16781677
  6. The protein interacts with the membrane as a functional monomer and the calculations confirm the presence of a membrane association domain that consists of layers of hydrophobic and positively charged residues. PMID: 17585909

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Subcellular Location
Mitochondrion inner membrane; Peripheral membrane protein; Matrix side.
Protein Families
Carnitine/choline acetyltransferase family
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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