Recombinant Rat Cell division cycle protein 20 homolog (Cdc20)

Code CSB-YP737086RA
MSDS
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Source Yeast
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Code CSB-EP737086RA
MSDS
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Source E.coli
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Code CSB-EP737086RA-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP737086RA
MSDS
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Source Baculovirus
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Code CSB-MP737086RA
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Cdc20
Uniprot No.
Alternative Names
Cdc20Cell division cycle protein 20 homolog; p55CDC
Species
Rattus norvegicus (Rat)
Expression Region
1-499
Target Protein Sequence
MAQFVFESDL HSLLQLDAPI PNAPIARWQR KAKEATGPAP SPMRAANRSH SAGRTPGRTP GKSNSKVQTT PSKPGGDRYI PQRSASQMEV ASFLLSKENQ PEDGGTPTKK EHQKAWARNL NGFDVEEAKI LRLSGKPQNA PEGYQNRLKV LYSQKATPGS SRKACRYIPS LPDRILDAPE IRNDYYLNLV DWSSGNVLAV ALDNSVYLWN AGSGDILQLL QMEQPGDYIS SVAWIKEGNY LAVGTSNAEV QLWDVQQQKR LRNMTSHSAR VSSLSWNSYI LSSGSRSGHI HHHDVRVAEH HVATLSGHSQ EVCGLRWAPD GRHLASGGND NIVNVWPSGP GESGWVPLQT FTQHQGAVKA VAWCPWQSNI LATGGGTSDR HIRIWNVCSG ACLSAVDVHS QVCSILWSPH YKELISGHGF AQNQLVIWKY PTMAKVAELK GHTARVLSLT MSPDGATVAS AAADETLRLW RCFELDPALR REREKASTSK SSLIHQGIR
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Required for full ubiquitin ligase activity of the anaphase promoting complex/cyclosome (APC/C) and may confer substrate specificity upon the complex. Is regulated by MAD2L1: in metaphase the MAD2L1-CDC20-APC/C ternary complex is inactive and in anaphase the CDC20-APC/C binary complex is active in degrading substrates. The CDC20-APC/C complex positively regulates the formation of synaptic vesicle clustering at active zone to the presynaptic membrane in postmitotic neurons. CDC20-APC/C-induced degradation of NEUROD2 induces presynaptic differentiation. Interacts with NEK2.
Gene References into Functions
  1. CDK2 blocks APC/CCdc20-mediated ubiquitylation of p21 at the N terminus, causing G2 arrest in mitogen-stimulated cardiomyocytes PMID: 22045811
  2. The functional roles of Cdc20 protein is involved in the cell cycle, mitosis, and apoptosis in CBRH-7919 cells. PMID: 20517887
  3. The expression level and activities of PC-PLC changed in a cell-cycle-dependent manner and were inversely correlated with the expression of Cdc20 PMID: 19347873
  4. findings define a Cdc20-APC(anaphase promoting complex) ubiquitin signaling pathway that governs presynaptic development, which holds important implications for neuronal connectivity and plasticity in the brain PMID: 19900895
Subcellular Location
Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle pole.
Protein Families
WD repeat CDC20/Fizzy family
Database Links
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Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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