Recombinant Rat Diacylglycerol kinase beta (Dgkb), partial

Code CSB-YP006833RA
MSDS
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Source Yeast
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Code CSB-EP006833RA
MSDS
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Source E.coli
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Code CSB-EP006833RA-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP006833RA
MSDS
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Source Baculovirus
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Code CSB-MP006833RA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Dgkb
Uniprot No.
Alternative Names
Dgkb; Dagk2; Diacylglycerol kinase beta; DAG kinase beta; EC 2.7.1.107; 90 kDa diacylglycerol kinase; Diglyceride kinase beta; DGK-beta
Species
Rattus norvegicus (Rat)
Expression Region
1-801
Target Protein Sequence
MTNQEKWAHL SPSEFSQLQK YAEYSTKKLK DVLEEFHGNG VLAKYNPEGK QDILNQTIDF EGFKLFMKTF LEAELPDDFT AHLFMSFSNK FPHSSPNVKS KPALLSGGLR MNKGAITPPR SSPANTCFPE VIHLKDIVCY LSLLERGRPE DKLEFMFRLY DTDGNGFLDS SELENIIGQM MHVAEYLEWD VTELNPILHE MMEEIDYDRD GTVSLEEWIQ GGMTTIPLLV LLGLENNVKD DGQHVWRLKH FNKPAYCNLC LNMLIGVGKQ GLCCSFCKYT VHERCARAPP SCIKTYVKSK KNTDVMHHYW VEGNCPTKCD KCHKTVKCYQ GLTGLHCVWC QTTLHNKCAS HLKPECDCGP LKDHILPPTT ICPVVLTMPT AGTSVPEERQ STAKKEKGSS QQPNKVTDKN KMQRANSVTM DGQGLQITPI PGTHPLLVFV NPKSGGKQGE RIYRKFQYLL NPRQVYSLSG NGPMPGLHFF RDVPDFRVLA CGGDGTVGWI LDCIEKANVV KHPPVAILPL GTGNDLARCL RWGGGYEGEN LMKILKDIES STEIMLDRWK FEVTPNDKDE KGDPVPYSII NNYFSIGVDA SIAHRFHIMR EKHPEKFNSR MKNKFWYFEF GTSETFSATC KKLHESVEIE CDGVQIDLIN ISLQGIAILN IPSMHGGSNL WGESKKKRSH RRIEKKGSDK RPTLTDAKEL KFASQDLSDQ LLEVVGLEGA MEMGQIYTGL KSAGRRLAQC SSVVIRTSKS LPMQIDGEPW MQTPCTIKIT HKNQAPMLMG PPPKTGLFCS LIKRTRNRSK E
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Diacylglycerol kinase that converts diacylglycerol/DAG into phosphatidic acid/phosphatidate/PA and regulates the respective levels of these two bioactive lipids. Thereby, acts as a central switch between the signaling pathways activated by these second messengers with different cellular targets and opposite effects in numerous biological processes (Probable). Has a higher activity with long-chain diacylglycerols like 1,2-di-(9Z-octadecenoyl)-sn-glycerol compared to 1,2-didecanoyl-sn-glycerol. Specifically expressed in brain, it regulates neuron-specific morphological changes including neurite branching and neurite spine formation.
Gene References into Functions
  1. This study demonistrated that dgkb distribuate in rat cerebellum. PMID: 23503970
  2. The DGKbeta localize in the outer plexiform layer, within which photoreceptor cells make contact with bipolar and horizontal cells. PMID: 23467923
  3. These results suggest that DGKepsilon and DGKzeta play distinct roles in the development of pressure overloaded cardiac hypertrophy and that the two isozymes are differentially regulated. PMID: 15896305
  4. In most brain regions, DGKbeta showed sustained expression throughout the postnatal developmental periods. And In the hippocampal pyramidal cell, DGKbeta localized along the membrane structure. PMID: 16019106
  5. Data suggest that abundant diacylglycerol kinase beta is present perisynaptically in medium spiny neurons so that it can effectively produce phosphatidic acid upon activation of Gq-coupled receptors and modulate striatal output neurons. PMID: 19087171
  6. In the hippocampus, DGKbeta is expressed in both projection neurons and interneurons and is accumulated at the perisynapse of dendritic spines in asymmetrical synapses. It may be involved in dendrite outgrowth and spinogenesis. PMID: 19691842

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Subcellular Location
Cell junction, synapse, postsynaptic cell membrane; Peripheral membrane protein. Cell membrane; Peripheral membrane protein. Cytoplasm.
Protein Families
Eukaryotic diacylglycerol kinase family
Tissue Specificity
Highly expressed in brain where it is restricted to neuronal populations such as the caudate-putamen, the accumbens nucleus, and the olfactory tubercle. Less abundantly expressed in adrenal gland, small intestine and heart.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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