Recombinant Rat Fasciculation and elongation protein zeta-1 (Fez1)

Code CSB-YP008600RA
MSDS
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Source Yeast
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Code CSB-EP008600RA
MSDS
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Source E.coli
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Code CSB-EP008600RA-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP008600RA
MSDS
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Source Baculovirus
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Code CSB-MP008600RA
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Fez1
Uniprot No.
Alternative Names
Fez1Fasciculation and elongation protein zeta-1; Zygin I; Zygin-1
Species
Rattus norvegicus (Rat)
Expression Region
1-393
Target Protein Sequence
MEAPLVSLDE EFEDIRPCCT EDPEEKPQSL YGTSPHHLED PSLSELENFS SEIISFKSME DLVNEFDEKL NVCFRNYNAK TENLAPVKNQ LQIQEEEETL RDEEVWDALT DNYIPSLSED WRDPNIEALN GNSSDTEIHE KEEEDEFIEK SENDSGINEE PLLTADQVIE EIEEMMQNSP DPEEEVEVLE EEDGGEISSQ ADSVLLQEMQ ALTQTFNNNW SYEGLRHMSG SELTELLDQV EGAIRDFSEE LVHQLARRDE LEFEKEVKNS FITVLIEVQN KQKEQRELMK KRRKEKGLSL QSSRIEKGNQ MPLKRFSMEG ISNILQSGIR QTFGSSGADR QYLNTVIPYE KKSSPPSVED LQMLTNILFA MKEDNEKVPT LLTDYILKVL CPT
Protein Length
Full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
May be involved in axonal outgrowth as component of the network of molecules that regulate cellular morphology and axon guidance machinery. May participate in the transport of mitochondria and other cargos along microtubules.
Gene References into Functions
  1. Phosphorylation of FEZ1 by MARK regulates its function in presynaptic protein trafficking. PMID: 27247180
  2. DISC1 participates in neurite outgrowth through its interaction with FEZ1. PMID: 12874605
  3. FEZ1 showed high levels of expression, especially in developing hippocampal neurons. PMID: 14992819
  4. E4B serves as a ubiquitin ligase for FEZ1 and thereby regulates its function but not its degradation PMID: 15466860
  5. FEZ1 promotes neurite extension through its interaction with microtubules, and agnoprotein facilitates JCV propagation by inducing the dissociation of FEZ1 from microtubules PMID: 15843383
  6. Overexpression of fasciculation and elongation protein zeta-1 (FEZ1) induces a post-entry block to retroviruses in cultured cells. PMID: 15879557
  7. Therefore, our results strongly suggest that FEZ1 participates in the establishment of neuronal polarity by controlling the mitochondrial motility along axon. PMID: 17173861
  8. the FEZ1/kinesin complex functions for the transport of mitochondria along microtubules toward the extending neurites in differentiating PC12 cells. PMID: 17669366
  9. Immunocytochemistry revealed that FEZ1 was located in the astrocytic cytoplasm and cell processes but not the nucleus PMID: 19199094
  10. FEZ1 appears to represent a unique neuron-specific determinant of cellular susceptibility to infection in a cell type that is naturally resistant to HIV-1 PMID: 19667186

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Subcellular Location
Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cell membrane.
Protein Families
Zygin family
Tissue Specificity
Brain.
Database Links
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