Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

Gpi

Uniprot No.

Q6P6V0

Research Area

Cancer

Alternative Names

(GPI)(Autocrine motility factor)(AMF)(Neuroleukin)(NLK)(Phosphoglucose isomerase)(PGI)(Phosphohexose isomerase)(PHI)

Species

Rattus norvegicus (Rat)

Source

Yeast

Expression Region

2-558aa

Target Protein Sequence

AALTRNPEFQKLLEWHRANSANLKLRELFEADPERFNHFSLNLNTNHGHILLDYSKNLVNKEVLHMLVDLAKSRGVEAARDNMFSGLKINSTEDRAVLHVALRNRSNRSIMMDGKDVMPEVNKVLDKMKSFCQRVRSGDWKGYTGKAITDIINIGIGGSDLGPLMVTEALKPYSKGGPRVWFVSNIDGTHIAKTLANLNPESSLFIIASKTFTTQETITNAETAKEWFLQAAKDPSAVAKHFVALSTNTDKVKEFGIDPKNMFEFWDWVGGRYSLWSAIGLSIALHVGFDHFEQLLSGAHWMDQHFMKTPLDKNAPVLLALLGIWYINFYGCETHAMLPYDQYMHRFAAYFQQGDMESNGKYITKSGARVDYQTGPIVWGEPGTNGQHAFYQLIHQGTKMIPCDFLIPVQTQHPIRNGLHHKILLANFLAQTEALMKGKSPEEARKELQAAGKSPEELEKLLPHKVFEGNRPTNSIVFTKLTPFILGALIAMYEHKIFVQGIIWDINSFDQWGVELGKQLAKKIEPELDGSSAVTSHDSSTNGLIGFIKLQRDTKID
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

63.8 kDa

Protein Length

Full Length of Mature Protein

Tag Info

C-terminal 6xHis-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

The production of the recombinant rat glucose-6-phosphate isomerase (Gpi) involves expressing a vector construct in yeast cells. This construct is created by cloning the gene fragment encoding the full-length mature rat Gpi protein (2-558aa) with a C-terminal 6xHis-tag into a plasmid vector. The resulting vector construct, containing the desired gene, is then transfected into the yeast cells. Special antibiotic selection is applied to choose the transfected yeast cells, which are subsequently cultured under suitable conditions. The induction reagents facilitate the expression of the recombinant rat Gpi protein within the yeast cells. Following expression, the yeast cells are harvested and lysed to release the cellular contents, including the recombinant Gpi protein. Purification of the recombinant Gpi protein is performed from the cell lysate, with its purity exceeding 90% as confirmed by SDS-PAGE analysis. On the gel, the recombinant Gpi protein appears as a band with a molecular weight of 64 kDa.

Target Background

Function

In the cytoplasm, catalyzes the conversion of glucose-6-phosphate to fructose-6-phosphate, the second step in glycolysis, and the reverse reaction during gluconeogenesis. Besides it's role as a glycolytic enzyme, also acts as a secreted cytokine: acts as an angiogenic factor (AMF) that stimulates endothelial cell motility. Acts as a neurotrophic factor, neuroleukin, for spinal and sensory neurons. It is secreted by lectin-stimulated T-cells and induces immunoglobulin secretion.

Gene References into Functions

Neuroleukin/Autocrine Motility Factor Receptor Pathway Promotes Proliferation of Articular Chondrocytes through Activation of AKT and Smad2/3 PMID: 26459914
induces hyperpermeability of endothelial and mesothelial cells leading to accumulation of ascites fluid (Tumor Autocrine Motility Factor) PMID: 12054583
Our results show that glucose-6-phosphate is not the only substrate of Glucose-6-Phosphate Isomerase. PMID: 16229142

Subcellular Location

Cytoplasm. Secreted.

Protein Families

GPI family

Database Links

KEGG: rno:292804

STRING: 10116.ENSRNOP00000029515

UniGene: Rn.84435

Code	CSB-YP744065RA
Abbreviation	Recombinant Rat Gpi protein
MSDS	MSDS Datasheet
Size	$436
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Recombinant Rat Glucose-6-phosphate isomerase (Gpi)

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Target Background

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