Recombinant Rat Inositol polyphosphate multikinase(Ipmk)

Code CSB-YP011774RA
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Source Yeast
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Code CSB-EP011774RA
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Source E.coli
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Code CSB-EP011774RA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP011774RA
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Source Baculovirus
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Code CSB-MP011774RA
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names Ipmk
Uniprot No. Q99NI4
Alternative Names Ipmk; ImpkInositol polyphosphate multikinase; EC 2.7.1.140; EC 2.7.1.151; EC 2.7.1.153; Inositol 1,3,4,6-tetrakisphosphate 5-kinase
Species Rattus norvegicus (Rat)
Expression Region 2-396
Target Protein Sequence AAEPPALRL RPPGSTGDSP PVPRLLGGCV PLSHQVAGHM YGKDKVGILQ HPDGTVLKQL QPPPRGPREL EFYTMVYAAD CADAVLLELR KHLPKYYGVW SPPSAPNDVY LKLEDVTHKF NKPCIMDVKI GRKSYDPFAS AEKIQQQVSK YPLMEEIGFL VLGMRVYHLH SDSYETQNQH YGRGLTKETL KEGVSKFFHN GFCLRKDAVA ASIQKVEKIL QWFENQKQLN FYASSLLFVY EGSSQPATTK SNDRTLAGRF LSKGALSDAD VLECNNNFHL FSSPANGTSV GKSLSKAYSR HRKLYAKKHQ SQTSLKVETL EQDNGWKSMS QEHLNGNVLS QLEKVFYHLP AGRQEIAEAE VRMIDFAHVF PSNTVDEGYV YGLKHLIAVL RSILDS
Protein Length Full Length of Mature Protein
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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Target Background

Function
Inositol phosphate kinase with a broad substrate specificity. Phosphorylates inositol 1,4,5-trisphosphate (Ins(1,4,5)P3) first to inositol 1,3,4,5-tetrakisphosphate and then to inositol 1,3,4,5,6-pentakisphosphate (Ins(1,3,4,5,6)P5). Phosphorylates inositol 1,3,4,6-tetrakisphosphate (Ins(1,3,4,6)P4). Phosphorylates glycero-3-phospho-1D-myo-inositol 4,5-bisphosphate to glycero-3-phospho-1D-myo-inositol 3,4,5-trisphosphate. Plays an important role in MLKL-mediated necroptosis via its role in the biosynthesis of inositol pentakisphosphate (InsP5) and inositol hexakisphosphate (InsP6). Binding of these highly phosphorylated inositol phosphates to MLKL mediates the release of an N-terminal auto-inhibitory region, leading to activation of the kinase. Essential for activated phospho-MLKL to oligomerize and localize to the cell membrane during necroptosis. Required for normal embryonic development, probably via its role in the biosynthesis of inositol 1,3,4,5,6-pentakisphosphate (Ins(1,3,4,5,6)P5) and inositol hexakisphosphate (InsP6).
Gene References into Functions
  1. Translocation of IPMK to plasma membrane via docking Dvl3 is important for IPMK to access substrates and generate IP5, activating down-stream signaling. PMID: 22940627
  2. IPK2 and IPK1 act as key determining steps in production of IP5 and IP6, respectively PMID: 15528195
Subcellular Location Nucleus.
Protein Families Inositol phosphokinase (IPK) family
Tissue Specificity Highly expressed in kidney, and at lower levels in hippocampus, brain cortex, cerebellum, heart and lung.
Database Links

KEGG: rno:171458

STRING: 10116.ENSRNOP00000000748

UniGene: Rn.15187

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Address
7505 Fannin St. Ste 610-312, Houston, TX 77054, USA
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