Recombinant Rat Phosducin (Pdc)

^{In Stock}

Code	CSB-EP017663RA
Abbreviation	Recombinant Rat Pdc protein
MSDS	MSDS Datasheet
Size	$224
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Product Details
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Target Background

Product Details

Purity

Greater than 85% as determined by SDS-PAGE.

Activity

Not Test

Target Names

Pdc

Uniprot No.

P20942

Research Area

Others

Species

Rattus norvegicus (Rat)

Source

E.coli

Expression Region

1-246aa

Target Protein Sequence

MEEAASQSLEEDFEGQATHTGPKGVINDWRKFKLESEDGDSIPPSKKEILRQMSSPQSRDDKDSKERMSRKMSIQEYELIHQDKEDEGCLRKYRRQCMQDMHQKLSFGPRYGFVYELETGEQFLETIEKEQKVTTIVVNIYEDGVRGCDALNSSLECLAAEYPMVKFCKIRASNTGAGDRFSSDVLPTLLVYKGGELISNFISVAEQFAEDFFAADVESFLNEYGLLPEREIHDLGQTNTEDEDIE
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

35.0 kDa

Protein Length

Full Length

Tag Info

C-terminal 6xHis-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function

Inhibits the transcriptional activation activity of the cone-rod homeobox CRX. May participate in the regulation of visual phototransduction or in the integration of photoreceptor metabolism.

Gene References into Functions

14-3-3 protein-mediated inhibition of Pdc binding to Gtbetagamma is based on steric occlusion of Pdc's Gtbetagamma binding surface. PMID: 28402877
C-terminal domain of Pdc interacts with the outside surface of the 14-3-3 dimer. PMID: 25971962
14-3-3 phosphorylation of Pdc at Ser-54 and Ser-73 affects the structure of the whole Pdc molecule. PMID: 23199924
data suggest that phosducin action is based on the reduction of transducin affinity to the membranes of rod outer segments, achieved by keeping the transducin beta gamma subunits apart from the alpha subunit PMID: 14973130
phosducin has a role in regulating signaling in the rod outer segment compartment and there are distinct functions for phosphorylation sites 54 and 71 PMID: 17569665

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Subcellular Location

Cytoplasm, cytosol. Nucleus. Cell projection, cilium, photoreceptor outer segment. Photoreceptor inner segment.

Protein Families

Phosducin family

Database Links

KEGG: rno:25343

STRING: 10116.ENSRNOP00000003405

UniGene: Rn.9739

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