Recombinant Rat Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2 (Hcn2), partial

Code CSB-YP881331RA
MSDS
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Source Yeast
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Code CSB-EP881331RA
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Source E.coli
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Code CSB-EP881331RA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP881331RA
MSDS
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Source Baculovirus
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Code CSB-MP881331RA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Hcn2
Uniprot No.
Alternative Names
Hcn2; Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2
Species
Rattus norvegicus (Rat)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Hyperpolarization-activated ion channel exhibiting weak selectivity for potassium over sodium ions. Contributes to the native pacemaker currents in heart (If) and in neurons (Ih). Can also transport ammonium in the distal nephron. Produces a large instantaneous current.
Gene References into Functions
  1. Hyperpolarization-activated cyclic nucleotide-gated (HCN) 2 protein expression levels were increased in the thalamus under chronic pain condition. Results suggest, increased HCN channel activity in the thalamus of the ascending nociceptive pathway contributes to both chronic neuropathic and inflammatory pain conditions. PMID: 27542339
  2. These results suggest that HCN2 in the subthalamic nucleus is involved in abnormal oscillation regulation between Motor cortex and globus pallidus . PMID: 25998542
  3. The results suggest that peripheral HCN2 channels contribute to mechanisms of spinal nerve injury-induced peripheral neuropathic pain. PMID: 25813712
  4. This study demonstrated that HCN2 contribute to heat hypersensitivity and ongoing activity in the neuritis model. PMID: 25290015
  5. Overexpression of HCN-2 channels in ventral tegmental area increases voluntary ethanol intake. PMID: 24460767
  6. Significant reductions in cardiac HCN2 mRNA and protein expression are demonstrated in genetic absence epilepsy rat-strain and acquired-epilepsy models. PMID: 24592881
  7. HCN2 channels contribute to inflammation-induced C-nociceptor hyperexcitability in chronic inflammatory pain. PMID: 22377439
  8. HCN2 band density in trigeminal ganglion neurons increased with postnatal age P1-P35. PMID: 21753027
  9. HCN2 is a basolateral ammonium transport pathway of intercalated cells and may contribute to the renal regulation of body pH under basal conditions. PMID: 21796099
  10. The results indicated that HCN2 ion channel mechanisms may play a role in SP-mediated spinal pain processing not only in naive animals but also in chronic inflammatory pain. PMID: 20726890
  11. HCN2 is distributed similarly to HCN1 along plasma membrane of dendritic shafts in layer I of the neocortex and stratum lacunosum moleculare of the hippocampal CA1 area. PMID: 14991560
  12. HCN2 widely co-localizes with calcitonin gene-related peptide, indicating HCN2 is expressed in peptidergic nociceptive primary afferents. Expression of HCN2 in terminals of peptidergic primary afferents was verified with electron microscopy. PMID: 15016091
  13. HCN2 staining was strongest in the MNTB and the dorsal nucleus of the lateral lemniscus. Strong HCN2 antibody labelling was also observed in bushy cells of the ventral cochlear nucleus. PMID: 15245481
  14. Mirpq channel acts as a beta subunit for HCN2 pacemaker channel subunits and alters channel gating at physiologically relevant voltages in cardiac cells. PMID: 15292247
  15. Consistent with the strong protein expression, an I(h) was recorded with features of an HCN2-mediated current in ST14A cells at the proliferative stage and in short-term-cultured E14 cells. PMID: 16175581
  16. expression levels of the HCN2 isoform found to be significantly higher in hypertrophied myocytes PMID: 16395601
  17. HCN-2 is the predominant subtype of HCN channels, which can control neuronal excitability, in small-sized C-fiber and medium-sized Adelta fiber DRG neurons including bladder afferent neurons. PMID: 16979600
  18. Reduction in HCN2 channel expression is an accompaniment of epileptogenesis in different adult models of temporal lobe epilepsy. PMID: 18397293
  19. HCN2 immunofluorescent staining was heterogeneous and predominantly in large diameter neurons and in small diameter IB4-negative neurons PMID: 18450385
  20. Overexpression of HCN2 channels blunts excitability of aortic baroreceptor neurons and baroreflex sensitivity in diabetic rats. PMID: 18524809
  21. Identify a four-amino acid motif (EEYP) in the B-helix of HCN2 that strongly promotes channel export from the endoplasmic reticulum and cell surface expression but does not contribute to the inhibition of channel opening. PMID: 18614814
  22. A loss of synchronous activity in the CA3 correlated with an increase in HCN2 mRNA levels that normalized concomitantly with the restoration of CA3 burst activity 7 days post insult. In PMID: 19500574

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Subcellular Location
Cell membrane; Multi-pass membrane protein.
Protein Families
Potassium channel HCN family
Tissue Specificity
Highly expressed in neonatal and adult ventricle and in brain. Highly expressed in the pyramidal layer in hippocampus, in anterior dorsal nucleus in thalamus, in the mammillary nucleus in hypothalamus, in red nucleus, in trigeminal mesencephalic, spinal a
Database Links
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