Recombinant Rat Protein AATF (Aatf)

Code CSB-YP874132RA
MSDS
Size Pls inquire
Source Yeast
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Code CSB-EP874132RA
MSDS
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Source E.coli
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Code CSB-EP874132RA-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP874132RA
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP874132RA
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Aatf
Uniprot No.
Alternative Names
AatfProtein AATF; Apoptosis-antagonizing transcription factor
Species
Rattus norvegicus (Rat)
Expression Region
2-523
Target Protein Sequence
AELQPLALQ LEQLLNPRPR EADPEADPEE ATRARVIDRF DEGEEEEDDL PVSSIRKLAP VSLLDTDKRY SGKTTSRKAW KEDHWDQTLP SSSDNEIPDE GGSEAGDSEG LEELSEDVEE DLEDNEIPDE GGSEDGDSEG LEEEISEDVE EDLEGEDEED REEDRNSEDD GVVMAFSGVK VSEEVEKGRA VKNQIALWDQ LLEGRIKLQK ALLTTNQLPQ PDVFPVFKDK GGPEFASALK NSHKALKALL RSLVDLQEEL LFQYPDTRYL VKGTKPNAES EEISSEDDEL VGEKKKQRKA PPKRKLEMED YPSFMAKRSA DFTVYRNRTL QKWHDKTKLA SGKLGKGFGA FERSILTQID HILMDKERLL RRTQTKRSAY RVLGKPEPVP EPVAETLPGE PESLPQVPAN AHLKDLDEEI FDDDDFYHQL LRELIERKTS SLDPNDQVAM GRQWLAIQKL RSKIRKKVDR KASKGRKLRF HVLSKLLSFM APIDHTAMND EARTELYRSL FGQLNRLDAD HGQ
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
May function as a general inhibitor of the histone deacetylase HDAC1. Binding to the pocket region of RB1 may displace HDAC1 from RB1/E2F complexes, leading to activation of E2F target genes and cell cycle progression. Conversely, displacement of HDAC1 from SP1 bound to the CDKN1A promoter leads to increased expression of this CDK inhibitor and blocks cell cycle progression. May negatively regulate the pro-apoptotic activity of DAPK3/ZIPK. Can act cooperatively with TSG101 to stimulate AR mediated transcription activation. May negatively regulate the pro-apoptotic activity of DAPK3/ZIPK. Can act cooperatively with TSG101 to stimulate AR mediated transcription activation.
Gene References into Functions
  1. Che-1 plays an important role in neuronal apoptosis following traumatic brain injury. PMID: 23007641
  2. Ectopic expression of AATF or a constitutively active form of AKT1 confers on cells resistance to ER stress-mediated cell death, whereas RNAi-mediated knockdown of AATF or AKT1 renders cells sensitive to ER stress. PMID: 19911006
  3. apoptosis-antagonizing transcription factor binds to TSG101 in a process that enhances androgen receptor-mediated transcription by promoting its monoubiquitination PMID: 14761944
  4. In search of novel interaction partners of AATF, we identified BLOS2 (BLOC1S2, also termed Ceap) from a rat cDNA library. PMID: 17552904
Subcellular Location
Nucleus.
Protein Families
AATF family
Tissue Specificity
Ubiquitously expressed.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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