Recombinant Rat Protein disulfide-isomerase A3 (Pdia3)

Code CSB-YP017720RA
MSDS
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Source Yeast
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Code CSB-EP017720RA
MSDS
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Source E.coli
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Code CSB-EP017720RA-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP017720RA
MSDS
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Source Baculovirus
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Code CSB-MP017720RA
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Pdia3
Uniprot No.
Alternative Names
Pdia3; Erp60; Grp58; Protein disulfide-isomerase A3; EC 5.3.4.1; 58 kDa glucose-regulated protein; 58 kDa microsomal protein; p58; Disulfide isomerase ER-60; Endoplasmic reticulum resident protein 57; ER protein 57; ERp57; Endoplasmic reticulum resident protein 60; ER protein 60; ERp60; HIP-70; Q-2
Species
Rattus norvegicus (Rat)
Expression Region
25-505
Target Protein Sequence
SDVLEL TDENFESRVS DTGSAGLMLV EFFAPWCGHC KRLAPEYEAA ATRLKGIVPL AKVDCTANTN TCNKYGVSGY PTLKIFRDGE EAGAYDGPRT ADGIVSHLKK QAGPASVPLR TEDEFKKFIS DKDASVVGFF RDLFSDGHSE FLKAASNLRD NYRFAHTNVE SLVKEYDDNG EGITIFRPLH LANKFEDKIV AYTEKKMTSG KIKKFIQESI FGLCPHMTED NKDLIQGKDL LTAYYDVDYE KNTKGSNYWR NRVMMVAKTF LDAGHKLNFA VASRKTFSHE LSDFGLESTT GEIPVVAIRT AKGEKFVMQE EFSRDGKALE RFLQEYFDGN LKRYLKSEPI PETNEGPVKV VVAESFDDIV NAEDKDVLIE FYAPWCGHCK NLEPKYKELG EKLSKDPNIV IAKMDATAND VPSPYEVKGF PTIYFSPANK KLTPKKYEGG RELNDFISYL QREATNPPII QEEKPKKKKK AQEDL
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Gene References into Functions
  1. VDR and PDIA3/1,25MARRS genes were silenced separately or simultaneously in E16 primary rat cortical neurons PMID: 28707894
  2. Proteomic data revealed significant Pdia-3 expression in microenvironments of osteoarthritis (OA) joint tissue that could be actively responded to extracorporeal shockwave therapy, which may potentially regulate the biological functions of chondrocytes and osteoblasts in the treatment of the early OA of the knee. PMID: 29104478
  3. it is possible that the vimentin and PDIA3 could be the candidate biomarkers specific to Achyranthes saponin therapy for rheumatoid arthritis in synovial membrane. PMID: 26724776
  4. In Atg7 knock-down INS-1 cells, the suppression of ERp57 expression by siRNA resulted in an increase in the level of cleaved Caspase-3 protein and a decrease in the number of live cells. PMID: 25241190
  5. ERp57 increased between MI and MII oocytes, and concentrated in a dome-shaped area of the microvillar region. Sperm ERp57 localized to the equatorial region of acrosome-reacted sperm. It was needed for penetrating zona pellucida-free oocytes. PMID: 24415168
  6. The results provide some primary information about PDIA3 in testis and sperm for further study on PDIA3 function in rat spermatogenesis and sperm-egg fusion. PMID: 23317155
  7. ERp57 regulates RalA signalling by acting as a redox-sensitive guanine-nucleotide dissociation inhibitor. PMID: 23226417
  8. Results suggest that the charge of amino acid especially, H258, is important for the BPA to bind to protein disulphide isomerase (PDI). PMID: 21976707
  9. Results suggest that PDIA3 and hepsin undergo proteolysis by MMP2/9 in ovarian granulosa cells during ovulation and/or luteinization. PMID: 21734266
  10. GRp58 may act as a molecular chaperone that alters the protein conformation of RARA for its delivery to the nucleus and, then with time, accompanies RARA to the ER for RARA ubiquitination and proteasome-mediated ERAD. PMID: 20130111
  11. Interaction with grp58 increases activity of the thiazide-sensitive Na-Cl cotransporter. PMID: 11832422
  12. The oxidoreductase ERp57 efficiently reduces partially folded in preference to fully folded MHC class I molecules. (ERp57) PMID: 12032078
  13. The ERp57-tapasin conjugate can also be modified with the oxidizing agent diamide, indicating that within the pool of ERp57-tapasin complexes the free, non-tapasin-linked CXXC motif exists in both oxidized and reduced states PMID: 13678524
  14. 1,25D3-MARRS has a role in steroid stimulated calcium uptake [review] PMID: 15862830
  15. 1,25D3-MARRS protein and vitamin D receptor work together to fine tune intestinal Ca2+ absorption PMID: 15862831
  16. demonstrate an optimum spacing within the redox-active motif of ERp57 PMID: 16516209
  17. Chondrocytes secrete 1,25(OH)(2)D(3), which acts directly on matrix vesicles-membranes via ERp60, releasing MMPs. PMID: 18765931

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Subcellular Location
Endoplasmic reticulum. Endoplasmic reticulum lumen. Melanosome.
Protein Families
Protein disulfide isomerase family
Tissue Specificity
In caput epididymal spermatozoa, detected in the head, mid and principal pieces. In cauda epididymal spermatozoa detected only in the acrosome (at protein level).
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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