Recombinant Rat Tripartite motif-containing protein 55 (Trim55)

Code CSB-YP735558RA
MSDS
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Source Yeast
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Code CSB-EP735558RA
MSDS
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Source E.coli
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Code CSB-EP735558RA-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP735558RA
MSDS
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Source Baculovirus
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Code CSB-MP735558RA
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Trim55
Uniprot No.
Alternative Names
Trim55; Murf2; Rnf29; Tripartite motif-containing protein 55; Muscle-specific RING finger protein 2; MuRF-2; MuRF2; RING finger protein 29
Species
Rattus norvegicus (Rat)
Expression Region
1-545
Target Protein Sequence
MSTSLNYKSF SKEQQTMDNL EKQLICPICL EMFTKPVVIL PCQHNLCRKC ASDIFQASNP YLPTRGGTTV ASGGRFRCPS CRHEVVLDRH GVYGLQRNLL VENIIDIYKQ ESTRPEKKLD QPMCEEHEEE RINIYCLNCE VPTCSLCKVF GAHKDCQVAP LTHVFQRQKS ELSDGIAVLV GSNDRVQGVI SQLEDTCKTI EECCRKQKQD LCEKFDHLYS ILEERKTEMT QAITRTQEEK LEHVRTLIRK YSDHLENVSK LVESGIQFMD EPEMAVFLQN AKTLLQKITE ASKAFQMEKI EQGYEIMNNF TVNLNREEKI IREIDFSREE EDEDDEGEVD EEGEGEDAVE VEEAENVQVA SSGEEETLEK AAEPSELAAE IQAASGPLLA SSPDSVSSLP PAADVLVTQG EVVPTDSQQT TQSETSGPSA AETADPLFYP SWYKGQSRKM ISNPPHTPGG EGLSQIGPSA AEDSSVQSAE VAEAAANEQA AVSGKESSST AATSQIGFEA SSPQGQAAAL GSGGGADSEP ARHVFSFSWL NSLNE
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
May regulate gene expression and protein turnover in muscle cells.
Gene References into Functions
  1. Neonatal rat cardiomyocytes expressing MURF2+3 specific siRNA have reduced expression of both MURF2 and -3 PMID: 21185285
Subcellular Location
Cytoplasm. Nucleus. Note=Nuclear under atrophic conditions and upon mechanical signals. Localizes to the sarcomeric M-band in cardiomyocytes. Colocalizes in part with microtubules.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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