Recombinant Rat Wilms tumor protein homolog(Wt1)

Code CSB-YP026158RA
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Source Yeast
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Code CSB-EP026158RA
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Source E.coli
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Code CSB-EP026158RA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP026158RA
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Source Baculovirus
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Code CSB-MP026158RA
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names Wt1
Uniprot No. P49952
Alternative Names Wt1; Wt-1Wilms tumor protein homolog
Species Rattus norvegicus (Rat)
Expression Region 1-448
Target Protein Sequence MGSDVRDLNA LLPAVSSLGG GGGCGLPVSG ARQWAPVLDF APPGASAYGS LGGPAPPPAP PPPPPPPHSF IKQEPSWGGA EPHEEQCLSA FTLHFSGQFT GTAGACRYGP FGPPPPSQAS SGQARMFPNA PYLPSCLESQ PSIRNQGYST VTFDGAPSYG HTPSHHAAQF PNHSFKHEDP MGQQGSLGEQ QYSVPPPVYG CHTPTDSCTG SQALLLRTPY SSDNLYQMTS QLECMTWNQM NLGATLKGMA AGSSSSVKWT EGQSNHGTGY ESENHTTPIL CGAQYRIHTH GVFRGIQDVR RVSGVAPTLV RSASETSEKR PFMCAYPGCN KRYFKLSHLQ MHSRKHTGEK PYQCDFKDCE RRFSRSDQLK RHQRRHTGVK PFQCKTCQRK FSRSDHLKTH TRTHTGKTSE KPFSCRWHSC QKKFARSDEL VRHHNMHQRN MTKLHVAL
Protein Length Full length protein
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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Target Data

Function Transcription factor that plays an important role in cellular development and cell survival. Recognizes and binds to the DNA sequence 5'-GCG(T/G)GGGCG-3'. Regulates the expression of numerous target genes, including EPO. Plays an essential role for development of the urogenital system. It has a tumor suppressor as well as an oncogenic role in tumor formation. Function may be isoform-specific
Gene References into Functions
  1. this study shows that WT1 ameliorates podocyte injury via repression of EZH2/beta-catenin pathway in diabetic nephropathy PMID: 28315733
  2. Renal WT1 expression is decreased in spontaneously hypertensive rats before the development of hypertension. PMID: 27009470
  3. High WT1 protein is associated with reduced ability to maintain podocyte integrity, rendering intrauterine growth restriction rats more susceptible for renal disease. PMID: 23229934
  4. ATRA increases the number of glomerular epithelial transition cells in experimental proteinuric glomerular diseases. Thus, ATRA may provide a useful pharmacologic approach to decipher the PMID: 23107969
  5. report of inhibition of FSHRexpression byWT1in ovarian tissue, and inhibition probably occurs via direct interaction with the FSHR promoter PMID: 22431408
  6. The re-expression of WT1 and Pax2 in the tubular epithelial cells plays important roles in the promotion of epithelial-mesenchymal transition. PMID: 21778682
  7. Downregulation of diaphragmatic WT1 gene expression may impair diaphragmatic development in the nitrofen CDH model. PMID: 21072664
  8. hypoxia upregulation of Wt1 expression involves hypoxia-inducible factor-1. PMID: 12738801
  9. Vitamin A deficiency can increase development of nephroblastomas in rats, probably via a mechanism of elevated nephrogenic rests remnants and WT1 expression PMID: 16338327
  10. genome-wide screen implicates WT1 in the transcriptional regulation of the EGF-family of growth factors as well as the CX3CL1 chemokine during nephrogenesis. PMID: 17430890
  11. Upregulation of B7.1 with the downregulation of GLEPP1, Wilms' tumor gene (WT1), megalin, and vascular endothelial growth factor started early and persisted through the course of primary glomercular disease. PMID: 17457373
  12. Wt1 modulates renin gene transcription. PMID: 18496514

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Subcellular Location Isoform 1: Nucleus speckle, SUBCELLULAR LOCATION: Isoform 4: Nucleus, nucleoplasm, SUBCELLULAR LOCATION: Nucleus, Nucleus, nucleolus, Cytoplasm, Nucleus speckle
Protein Families EGR C2H2-type zinc-finger protein family
Tissue Specificity Kidney.
Database Links

KEGG: rno:24883

STRING: 10116.ENSRNOP00000060038

UniGene: Rn.92531

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