Recombinant Rat cGMP-specific 3',5'-cyclic phosphodiesterase (Pde5a), partial

Code CSB-YP523851RA
Size Pls inquire
Source Yeast
Have Questions? Leave a Message or Start an on-line Chat
Code CSB-EP523851RA
Size Pls inquire
Source E.coli
Have Questions? Leave a Message or Start an on-line Chat
Code CSB-EP523851RA-B
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
Have Questions? Leave a Message or Start an on-line Chat
Code CSB-BP523851RA
Size Pls inquire
Source Baculovirus
Have Questions? Leave a Message or Start an on-line Chat
Code CSB-MP523851RA
Size Pls inquire
Source Mammalian cell
Have Questions? Leave a Message or Start an on-line Chat

Product Details

Purity
>85% (SDS-PAGE)
Target Names
Pde5a
Uniprot No.
Alternative Names
Pde5a; Pde5; cGMP-specific 3',5'-cyclic phosphodiesterase; EC 3.1.4.35; cGMP-binding cGMP-specific phosphodiesterase; CGB-PDE
Species
Rattus norvegicus (Rat)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

 Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Target Background

Function
Plays a role in signal transduction by regulating the intracellular concentration of cyclic nucleotides. This phosphodiesterase catalyzes the specific hydrolysis of cGMP to 5'-GMP. Specifically regulates nitric-oxide-generated cGMP.
Gene References into Functions
  1. Our data showed a significant and previously undocumented upregulation of PDE5 in both rat and human BPH. PMID: 26657792
  2. The effects of vasodilation on plasma volume expansionhemodilution, and PDE5A expression via the renin-angiotensin-aldosterone system are reported. PMID: 24068049
  3. The phosphodiesterase 5A inhibitors decrease NHE-1 phosphorylation and activity through a mechanism that involves PP2A activation. PMID: 21063091
  4. mRNA transcript and protein levels of PDE5 in the main and first branch of the pulmonary arteries are increased by chronic hypoxia. PMID: 12466227
  5. PDE-5 is constitutively present in the rat uterine wall. There was no observed change in the PDE-5 protein expression throughout pregnancy. PMID: 14749671
  6. Kinetics of rat cellular nitric oxide/cGMP/phosphodiesterase-5 pathway PMID: 15075333
  7. normal rat pregnancy leads to in vivo and in vitro renal resistance to atrial natriuretic peptide, in association with heightened activity of the cGMP-specific PDE5 PMID: 15100365
  8. PDE5A may also play a critical role in the growth promoting effects of Angiotensin II PMID: 15623434
  9. Increased PDE-5 expression is involved in the decreased vascular response to nitric oxide in cirrhotic livers PMID: 16545481
  10. PDE5 controls the soluble cyclic GMP pool in cardiac myocytes. PMID: 16651469
  11. PDE5 activity limits action potential propagation in neurohypophysial axons. PMID: 17690141
  12. Phosphodiesterase 5A expression confirms the activity targeted inhibition by sildenafil in cardiomyocytes and hypertrophy modulation. PMID: 18790048
  13. PDE5 expression was not under direct androgen regulation. PMID: 19338748

Show More

Hide All

Protein Families
Cyclic nucleotide phosphodiesterase family
Database Links
icon of phone
Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
icon of address
Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
icon of social media
Join us with

Subscribe newsletter

Leave a message

* To protect against spam, please pass the CAPTCHA test below.
CAPTCHA verification
© 2007-2024 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1
webinars: DT3C facilitates antibody internalization X