Recombinant Saccharomyces cerevisiae Histone demethylase JHD2 (JHD2), partial

Code CSB-YP344439SVG
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Source Yeast
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Code CSB-EP344439SVG
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Source E.coli
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Code CSB-EP344439SVG-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP344439SVG
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Source Baculovirus
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Code CSB-MP344439SVG
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
JHD2
Uniprot No.
Alternative Names
JHD2; YJR119C; J2035Histone demethylase JHD2; EC 1.14.11.-; Jumonji/ARID domain-containing protein 2
Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Histone demethylase that demethylates 'Lys-4' of histone H3, thereby playing a central role in histone code. Demethylates trimethylated H3 'Lys-4'.
Gene References into Functions
  1. H2B Lys-123 ubiquitination blocks Jhd2 from accessing its binding site on chromatin, and thereby, we have uncovered a second mechanism by which H2B ubiquitination contributes to the trans-histone regulation of H3K4 methylation. PMID: 26451043
  2. Jhd2, an evolutionarily conserved JARID1 family H3 Lys4 demethylase, effects all states of methylated histone H3K4 within the NTS regions of rDNA, and its activity is required for the regulation of rDNA silencing in a Sir2-independent manner. PMID: 25248920
  3. the JmjN domain of Jhd2 is important for its protein stability, and the plant homeodomain (PHD) finger mediates its chromatin association independent of H3K4 methylation PMID: 20538609
  4. A novel budding yeast JmjC domain-containing H3-K4 demethylase, Jhd2p, that antagonizes the trimethyl modification state and contributes to regulation of telomeric silencing. PMID: 17310254
  5. Ectopic overexpression of mouse Jarid1B, a Yjr119Cp homolog, greatly diminished H3-(lysine) K4me3 and H3-K4me2 in HeLa cells, suggesting these proteins function as K4 demethylases in vivo. PMID: 17310255
  6. the protein level of the yeast histone H3 Lys 4 (H3 K4) demethylase Jhd2/Kdm5 is modulated through polyubiquitination by the E3 ubiquitin ligase Not4 and turnover by the proteasome PMID: 19346402

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Subcellular Location
Nucleus.
Protein Families
JARID1 histone demethylase family
Database Links

KEGG: sce:YJR119C

STRING: 4932.YJR119C

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