Recombinant Saccharomyces cerevisiae ADP-ribosylation factor-like protein 1 (ARL1)

Code CSB-YP002070SVG
MSDS
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Source Yeast
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Code CSB-EP002070SVG
MSDS
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Source E.coli
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Code CSB-EP002070SVG-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP002070SVG
MSDS
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Source Baculovirus
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Code CSB-MP002070SVG
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
ARL1
Uniprot No.
Alternative Names
ARL1; ARF3; YBR164C; YBR1216ADP-ribosylation factor-like protein 1; Arf-like GTPase 1
Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Expression Region
2-183
Target Protein Sequence
GNIFSSMFD KLWGSNKELR ILILGLDGAG KTTILYRLQI GEVVTTKPTI GFNVETLSYK NLKLNVWDLG GQTSIRPYWR CYYADTAAVI FVVDSTDKDR MSTASKELHL MLQEEELQDA ALLVFANKQD QPGALSASEV SKELNLVELK DRSWSIVASS AIKGEGITEG LDWLIDVIKE EQL
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Recruits golgins such as IMH1 to the Golgi. Can bind and hydrolyze GTP. May be involved in trafficking events within the endosomal system.
Gene References into Functions
  1. Arl3-Arl1 cascade acts on the cytoplasm-to-vacuole targeting pathway via dynamic nucleotide binding PMID: 28627726
  2. Hac1p restored the Golgi localization of Arl1p inIRE1-deleted cells. Ire1p-Hac1p axis regulated Syt1p phosphorylation, which enhances Arl1p activation, recruitment of Imh1p to the Golgi, and Syt1p interaction with Arl1p. PMID: 26966233
  3. The authors demonstrated that MON2 is a negative regulator of the GTP-restricted allele of ARL1 , ARL1[Q72L]. PMID: 22594927
  4. gene deletions affecting the Arl1 vesicle-tethering pathways prevent Arl1 activation and membrane localization PMID: 21562219
  5. Findings reveal that activation of Arl1p is regulated in part by Syt1p, and imply that Arl1p activation, by using more than one GEF, exerts distinct biological activities at the Golgi compartment. PMID: 20841378
  6. results are consistent with a model in which ARL1, via regulation of HAL4/HAL5, governs K(+) homeostasis in cells PMID: 15126631
  7. Both ARF-like protein Arl1p and Gcs1p influence the distribution of Imh1p, an Arl1p effector. PMID: 15975906
  8. Arl1p is involved in the anterograde transport from the Golgi to the cell surface of the glycosylphosphatidylinositol (GPI)-anchored plasma-membrane-resident protein Gas1p. PMID: 16926193

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Subcellular Location
Golgi apparatus.
Protein Families
Small GTPase superfamily, Arf family
Database Links

KEGG: sce:YBR164C

STRING: 4932.YBR164C

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