Product Details

Purity

Greater than 85% as determined by SDS-PAGE.

Target Names

ACC1

Uniprot No.

Q00955

Research Area

Cancer

Alternative Names

ACC1; ABP2; FAS3; MTR7; YNR016C; N3175Acetyl-CoA carboxylase; ACC; EC 6.4.1.2; Fatty acid synthetase 3; mRNA transport-defective protein 7) [Includes: Biotin carboxylase; EC 6.3.4.14)]

Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Source

E.coli

Expression Region

2-581aa

Target Protein Sequence

SEESLFESSPQKMEYEITNYSERHTELPGHFIGLNTVDKLEESPLRDFVKSHGGHTVISKILIANNGIAAVKEIRSVRKWAYETFGDDRTVQFVAMATPEDLEANAEYIRMADQYIEVPGGTNNNNYANVDLIVDIAERADVDAVWAGWGHASENPLLPEKLSQSKRKVIFIGPPGNAMRSLGDKISSTIVAQSAKVPCIPWSGTGVDTVHVDEKTGLVSVDDDIYQKGCCTSPEDGLQKAKRIGFPVMIKASEGGGGKGIRQVEREEDFIALYHQAANEIPGSPIFIMKLAGRARHLEVQLLADQYGTNISLFGRDCSVQRRHQKIIEEAPVTIAKAETFHEMEKAAVRLGKLVGYVSAGTVEYLYSHDDGKFYFLELNPRLQVEHPTTEMVSGVNLPAAQLQIAMGIPMHRISDIRTLYGMNPHSASEIDFEFKTQDATKKQRRPIPKGHCTACRITSEDPNDGFKPSGGTLHELNFRSSSNVWGYFSVGNNGNIHSFSDSQFGHIFAFGENRQASRKHMVVALKELSIRGDFRTTVEYLIKLLETEDFEDNTITTGWLDDLITHKMTAEKPDPTLAV
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

69.9 kDa

Protein Length

Partial

Tag Info

N-terminal 10xHis-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

The Recombinant Saccharomyces cerevisiae ACC1 is an indispensable tool for those focusing on cancer research and exploring the pathways of lipid metabolism. This protein, sourced from the well-characterized Baker's yeast strain (ATCC 204508 / S288c), is an acetyl-CoA carboxylase, a pivotal enzyme involved in fatty acid biosynthesis. The in-depth study of this enzyme can offer profound insights into cellular energy metabolism, especially within the context of cancer.

This version of the ACC1 protein encompasses a partial sequence (2-581 amino acids) but retains critical functionality for a broad range of applications. Expressed in an E.coli system, it features an N-terminal 10xHis tag for efficient detection and purification. The quality is reflected in the purity greater than 85% as determined by SDS-PAGE, highlighting the robustness of our purification process. Available in liquid or lyophilized powder form, the Recombinant Saccharomyces cerevisiae ACC1 ensures accuracy, high quality, and dependable results for your research.

Target Background

Function

Carries out three functions: biotin carboxyl carrier protein, biotin carboxylase and carboxyltransferase. Involved in the synthesis of very-long-chain fatty acid synthesis which is required to maintain a functional nuclear envelope. Required for acylation and vacuolar membrane association of VAC8 which is necessary to maintain a normal morphology of the vacuole.

Gene References into Functions

Data show that attenuated expression of acetyl-CoA carboxylase ACC1 results in increased acetylation of bulk histones, globally increased acetylation of chromatin histones, and altered transcriptional regulation. PMID: 22580297

Subcellular Location

Cytoplasm. Endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side.

Database Links

KEGG: sce:YNR016C

STRING: 4932.YNR016C

Code	CSB-EP2725SVG2
Abbreviation	Recombinant Saccharomyces cerevisiae ACC1 protein, partial
MSDS	MSDS Datasheet
Size	$388
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Recombinant Saccharomyces cerevisiae Acetyl-CoA carboxylase (ACC1), partial

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