Recombinant Saccharomyces cerevisiae BNI1-related protein 1 (BNR1), partial

Code CSB-YP331171SVG
MSDS
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Source Yeast
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Code CSB-EP331171SVG
MSDS
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Source E.coli
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Code CSB-EP331171SVG-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP331171SVG
MSDS
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Source Baculovirus
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Code CSB-MP331171SVG
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
BNR1
Uniprot No.
Alternative Names
BNR1; YIL159WBNI1-related protein 1
Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
May organize microtubules by mediating spindle positioning and movement in the budding process. Potential target of the RHO family members.
Gene References into Functions
  1. Smy1 regulation of Bnr1 activity is required for efficient secretory vesicle traffic. PMID: 26764093
  2. F-actin stabilization is regulated by yeast formins Bni1p and Bnr1p PMID: 23653364
  3. Bnr1p has two separable regions that each contribute to its bud neck localization PMID: 20147448
  4. different formin isoforms have roles in maintaining stable and dynamic axes in the same yeast cell PMID: 15371545
  5. Bnr1 was confined to the bud neck and did not exchange with a cytoplasmic pool. PMID: 17344480
  6. Yeast expressing the nonlocalized actin nucleating/assembly formin homology (FH) 1-FH2 domains of Bnr1p or Bni1p as the sole formin grow well. PMID: 19297522

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Protein Families
Formin homology family, BNI1 subfamily
Database Links

KEGG: sce:YIL159W

STRING: 4932.YIL159W

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