Recombinant Saccharomyces cerevisiae Casein kinase I homolog HRR25 (HRR25)

Code CSB-YP341054SVG
MSDS
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Source Yeast
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Code CSB-EP341054SVG
MSDS
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Source E.coli
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Code CSB-EP341054SVG-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP341054SVG
MSDS
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Source Baculovirus
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Code CSB-MP341054SVG
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
HRR25
Uniprot No.
Alternative Names
HRR25; YPL204W; Casein kinase I homolog HRR25; EC 2.7.11.1
Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Expression Region
1-494
Target Protein Sequence
MDLRVGRKFR IGRKIGSGSF GDIYHGTNLI SGEEVAIKLE SIRSRHPQLD YESRVYRYLS GGVGIPFIRW FGREGEYNAM VIDLLGPSLE DLFNYCHRRF SFKTVIMLAL QMFCRIQYIH GRSFIHRDIK PDNFLMGVGR RGSTVHVIDF GLSKKYRDFN THRHIPYREN KSLTGTARYA SVNTHLGIEQ SRRDDLESLG YVLIYFCKGS LPWQGLKATT KKQKYDRIME KKLNVSVETL CSGLPLEFQE YMAYCKNLKF DEKPDYLFLA RLFKDLSIKL EYHNDHLFDW TMLRYTKAMV EKQRDLLIEK GDLNANSNAA SASNSTDNKS ETFNKIKLLA MKKFPTHFHY YKNEDKHNPS PEEIKQQTIL NNNAASSLPE ELLNALDKGM ENLRQQQPQQ QVQSSQPQPQ PQQLQQQPNG QRPNYYPEPL LQQQQRDSQE QQQQVPMATT RATQYPPQIN SNNFNTNQAS VPPQMRSNPQ QPPQDKPAGQ SIWL
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Associated with repair of damaged DNA and meiosis. Phosphorylates serine and threonine. Can use casein as a substrate.
Gene References into Functions
  1. The C-terminal region of Hrr25 is required for normal inhibition of expression of Haa1 target genes TPO2 and YRO2 and is important for cell growth but is not required for cell morphogenesis PMID: 28432100
  2. Together, the Hrr25 central domain and Mam1 form a novel, contiguous embellishment to the Hrr25 kinase domain that affects Hrr25 conformational dynamics and enzyme kinetics. PMID: 27491543
  3. Hrr25, a homolog of CSNK1D/casein kinase 1delta, regulates 3 of 4 selective autophagy-related pathways in the budding yeast Saccharomyces cerevisiae by a uniform mechanism: phosphoregulation of the receptor-scaffold interaction PMID: 25700828
  4. Hrr25 protein kinase directly modifies Elp1 on two sites and we provide evidence that phosphorylation plays a positive role in the tRNA modification PMID: 25569479
  5. Hrr25 is essential for ribosome assembly in Saccharomyces cerevisiae. PMID: 25778921
  6. Hrr25 is responsible for the phosphorylation of two receptor proteins: Atg19, which recognizes the assembly of vacuolar enzymes in the cytoplasm-to-vacuole targeting pathway, and Atg36, which recognizes superfluous peroxisomes in pexophagy. PMID: 25287303
  7. the kinase activity of Hrr25 regulates the maturation of 40S ribosomal subunits PMID: 16738661
  8. We show that monopolin subunit Mam1 binds tightly to Hrr25, a highly conserved casein kinase 1 delta/varepsilon, and recruits it to meiosis I centromeres. Hrr25 kinase activity and Mam1 binding are both essential for monopolar attachment. PMID: 16990132
  9. NUP53 is a target of HRR25. PMID: 17461799
  10. Hrr25p-mediated phosphorylation of Tif6p plays a critical role in the biogenesis of 60 S ribosomal subunits in yeast cells. PMID: 18256024

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Subcellular Location
Cytoplasm. Nucleus, nucleolus. Nucleus, nucleoplasm.
Protein Families
Protein kinase superfamily, CK1 Ser/Thr protein kinase family, Casein kinase I subfamily
Database Links

KEGG: sce:YPL204W

STRING: 4932.YPL204W

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