Recombinant Saccharomyces cerevisiae Cys-Gly metallodipeptidase DUG1 (DUG1)

Code CSB-YP331893SVG
MSDS
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Source Yeast
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Code CSB-EP331893SVG
MSDS
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Source E.coli
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Code CSB-EP331893SVG-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP331893SVG
MSDS
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Source Baculovirus
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Code CSB-MP331893SVG
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
DUG1
Uniprot No.
Alternative Names
DUG1; YFR044C; Cys-Gly metallodipeptidase DUG1; EC 3.4.13.-; Deficient in utilization of glutathione protein 1; GSH degradosomal complex subunit DUG1
Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Expression Region
1-481
Target Protein Sequence
MSHSLTSVFQ KIDSLKPQFF SRLTKAIQIP AVSSDESLRS KVFDKAKFIS EQLSQSGFHD IKMVDLGIQP PPISTPNLSL PPVILSRFGS DPSKKTVLVY GHYDVQPAQL EDGWDTEPFK LVIDEAKGIM KGRGVTDDTG PLLSWINVVD AFKASGQEFP VNLVTCFEGM EESGSLKLDE LIKKEANGYF KGVDAVCISD NYWLGTKKPV LTYGLRGCNY YQTIIEGPSA DLHSGIFGGV VAEPMIDLMQ VLGSLVDSKG KILIDGIDEM VAPLTEKEKA LYKDIEFSVE ELNAATGSKT SLYDKKEDIL MHRWRYPSLS IHGVEGAFSA QGAKTVIPAK VFGKFSIRTV PDMDSEKLTS LVQKHCDAKF KSLNSPNKCR TELIHDGAYW VSDPFNAQFT AAKKATKLVY GVDPDFTREG GSIPITLTFQ DALNTSVLLL PMGRGDDGAH SINEKLDISN FVGGMKTMAA YLQYYSESPE N
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Catalytic component of the GSH degradosomal complex involved in the degradation of glutathione (GSH) and other peptides containing a gamma-glu-X bond. Functions also in a DUG2-DUG3-independent manner as a dipeptidase with high specificity for Cys-Gly and no activity toward tri- or tetrapeptides.
Gene References into Functions
  1. Overexpression of cDNAs is a promising approach to engineer S. cerevisiae metabolism; here DUG1 overexpression lead to enhance growth of non-ethanol producing S. cerevisiae strains lacking pyruvate decarboxylases. PMID: 29636254
  2. Structural similarity between the Dug1p-peptide complex and the bestatin-bound complex of CN2 suggests that the Dug1p-peptide structure can be used as a template for designing natural peptide inhibitors. PMID: 25427234
  3. The Dug1p family represents a novel class of Cys-Gly dipeptidases. PMID: 19346245
Subcellular Location
Cytoplasm. Mitochondrion.
Protein Families
Peptidase M20A family
Database Links

KEGG: sce:YFR044C

STRING: 4932.YFR044C

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