Recombinant Saccharomyces cerevisiae Cysteine desulfurase, mitochondrial (NFS1)

Code CSB-YP326540SVG
MSDS
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Source Yeast
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Code CSB-EP326540SVG
MSDS
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Source E.coli
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Code CSB-EP326540SVG-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP326540SVG
MSDS
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Source Baculovirus
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Code CSB-MP326540SVG
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
NFS1
Uniprot No.
Alternative Names
NFS1; SPL1; YCL017C; Cysteine desulfurase; mitochondrial; EC 2.8.1.7; tRNA-splicing protein SPL1
Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Expression Region
34-497
Target Protein Sequence
YSPPAAG VKLDDNFSLE THTDIQAAAK AQASARASAS GTTPDAVVAS GSTAMSHAYQ ENTGFGTRPI YLDMQATTPT DPRVLDTMLK FYTGLYGNPH SNTHSYGWET NTAVENARAH VAKMINADPK EIIFTSGATE SNNMVLKGVP RFYKKTKKHI ITTRTEHKCV LEAARAMMKE GFEVTFLNVD DQGLIDLKEL EDAIRPDTCL VSVMAVNNEI GVIQPIKEIG AICRKNKIYF HTDAAQAYGK IHIDVNEMNI DLLSISSHKI YGPKGIGAIY VRRRPRVRLE PLLSGGGQER GLRSGTLAPP LVAGFGEAAR LMKKEFDNDQ AHIKRLSDKL VKGLLSAEHT TLNGSPDHRY PGCVNVSFAY VEGESLLMAL RDIALSSGSA CTSASLEPSY VLHALGKDDA LAHSSIRFGI GRFSTEEEVD YVVKAVSDRV KFLRELSPLW EMVQEGIDLN SIKWSGH
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Catalyzes the removal of elemental sulfur from cysteine to produce alanine. It supplies the inorganic sulfur for iron-sulfur (Fe-S) clusters. Plays a role in both tRNA-processing and mitochondrial metabolism. Involved in the 2-thio-modification of both 5-carboxymethylaminomethyl-2-thiouridine in mitochondrial tRNAs and 5-methoxycarbonylmethyl-2-thiouridine (mcm5s2U) in cytoplasmic tRNAs.
Gene References into Functions
  1. Compared with wild-type mitochondria, yck2 mitochondria revealed slower persulfide formation on Nfs1 consistent with a role of Yck2 in regulating mitochondrial cysteine desulfurase activity. PMID: 28941588
  2. Isu is a substrate of the Lon-type protease and that it is protected from degradation by Nfs1. Nfs1 variants known to be defective in interaction with Isu were also defective in protecting Isu from degradation. PMID: 26842892
  3. Mutual exclusivity of cluster assembly factor (Nfs1/Yfh1) and cluster transfer factor (Jac1/Ssq1) binding to Isu has functional consequences for the transition from the assembly process to the transfer process during biogenesis of FeS cluster proteins. PMID: 25228696
  4. The data presented here show that the Isu1 suppressor mimics the frataxin effects on Nfs1, explaining the bypassing activity. PMID: 24217246
  5. results show that Nfs1 adopts a higher oligomeric state in the Isd11-bound form compared to that in the free form PMID: 24045011
  6. Competition between Jac1 and Nfs1 for Isu binding plays an important role in transitioning the iron/Sulfur cluster biogenesis machinery. PMID: 23946486
  7. Thus the Nfs1p-bound persulfide as described here represents a precursor en route to Fe-S cluster synthesis in mitochondria. PMID: 22813754
  8. Whereas Nfs1p by itself was inactive, the Nfs1p.Isd11p complex formed persulfide and was active as a cysteine desulfurase. In the absence of Isd11p, Nfs1p was able to bind the substrate cysteine but failed to form a persulfide. PMID: 22928949
  9. Isd11 acts together with Nfs1 in an early step in the biogenesis of Fe/S proteins. PMID: 16341090
  10. Results are consistent with the existence of a mechanism to increase the stability of Isu, and thus its level, that is dependent on the presence of the cysteine desulfurase Nfs1. PMID: 18843040

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Subcellular Location
Mitochondrion.
Protein Families
Class-V pyridoxal-phosphate-dependent aminotransferase family, NifS/IscS subfamily
Database Links

KEGG: sce:YCL017C

STRING: 4932.YCL017C

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