Product Details

Purity

>85% (SDS-PAGE)

Target Names

MSH3

Uniprot No.

P25336

Alternative Names

MSH3; YCR092C; YCR1152; YCR92C; DNA mismatch repair protein MSH3; Mismatch-binding protein; MBP; MutS protein homolog 3

Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Target Background

Function

Component of the post-replicative DNA mismatch repair system (MMR). Heterodimerizes with MSH2 to form MutS beta, which binds to DNA mismatches thereby initiating DNA repair. MSH3 provides substrate-binding and substrate specificity to the complex. When bound, the MutS beta heterodimer bends the DNA helix and shields approximately 20 base pairs. Acts mainly to repair insertion-deletion loops (IDLs) from 2 to 13 nucleotides in size, but can also repair base-base and single insertion-deletion mismatches that occur during replication. After mismatch binding, forms a ternary complex with either the MutL alpha or MutL beta heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. MutS beta also has a role in regulation of heteroduplex formation during mitotic and meiotic recombination. MutS beta binds to DNA flap structures predicted to form during recombination, and is required for 3' non-homologous tail removal (NHTR). MutS beta-binding alters the DNA conformation of its substrate at the ds/ssDNA junction and may facilitate its recognition and/or cleavage by the downstream nucleotide excision repair (NER) RAD1-RAD10 endonuclease. ATP binding and hydrolysis play a pivotal role in MMR and NHTR.

Gene References into Functions

Msh2-Msh3 hops over nucleosomes and other protein roadblocks, but maintains sufficient contact with DNA to recognize a single lesion. PMID: 26837705
Trinucleotide Repeat (TNR) tracts were stabilized in the msh3Delta background, indicating that Msh2-Msh3 plays important role in shifting expansion-contraction equilibrium toward expansion in early stages of TNR tract expansion. PMID: 25969461
Data suggest that bot hATP and structure-specific repair substrates cooperate to direct Msh2-Msh3-mediated repair and provide an explanation for the msh3Y942A separation-of-function phenotype. PMID: 24746922
a comparative study of Msh2-Msh3 and Msh2-Msh6 for mispair binding, sliding clamp formation, and Mlh1-Pms1 recruitment PMID: 24550389
Mlh1-Mlh3, a meiotic crossover and DNA mismatch repair factor, is a Msh2-Msh3-stimulated endonuclease. PMID: 24403070
Authors identified a set of aromatic residues within the FLY motif of the predicted Msh3 nucleotide binding pocket that are essential for Msh2-Msh3-mediated mismatch repair but are largely dispensable for 3' non-homologous tail removal. PMID: 23458407
Msh2-Msh3 directly interferes with normal Okazaki fragment processing by flap endonuclease1 (Rad27) and DNA ligase I (Cdc9) in the presence of TNR sequences, thereby producing small, incremental expansion events PMID: 22938864
Data suggest that recognition of small insertion/deletion mispairs by Msh3, but not Msh2, requires more interaction with DNA conformations induced by small insertion/deletion mispairs than induced by large that are bent and strand separated. PMID: 20421420
MSH2-MSH3 binding to its substrates creates a unique nucleoprotein structure that may signal downstream steps in repair that include interactions with mismatch repair (MMR) and nucleotide excision repair factors. PMID: 16781730
The binding of MSH2-MSH3 to mismatch DNA involves protein-DNA contacts that appear very different from those required for MSH2-MSH6 mismatch binding. PMID: 17157869
Msh3-like behaviors beyond mispair specificity are not features controlled by the mispair-binding domain PMID: 17573527
MSH3 acts in repair of base pair mismatch. PMID: 17636021

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Subcellular Location

Nucleus.

Protein Families

DNA mismatch repair MutS family, MSH3 subfamily

Database Links

KEGG: sce:YCR092C

STRING: 4932.YCR092C

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Recombinant Saccharomyces cerevisiae DNA mismatch repair protein MSH3 (MSH3), partial

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