Product Details

Purity

>85% (SDS-PAGE)

Target Names

RAD5

Uniprot No.

P32849

Alternative Names

RAD5; REV2; SNM2; YLR032W; DNA repair protein RAD5; EC 3.6.4.-; Radiation sensitivity protein 5; Revertibility protein 2

Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Target Background

Function

Probable helicase, member of the UBC2/RAD6 epistasis group. Functions with the DNA repair protein RAD18 in error-free postreplication DNA repair. Involved in the maintenance of wild-type rates of instability of simple repetitive sequences such as poly(GT) repeats. Seems to be involved in maintaining a balance which acts in favor of error-prone non-homologous joining during DNA double-strand breaks repairs. Recruits the UBC13-MMS2 dimer to chromatin for DNA repair.

Gene References into Functions

By analyzing the formation of Rad5 foci after MMS treatment, we showed that some specific DNA structures rather than mono-ubiquitination of proliferating cell nuclear antigen are required for the recruitment of Rad5 to the damaged site PMID: 29396601
Rad5 Ub-ligase and ATPase/helicase activities are required for the response to DNA damage. PMID: 25310987
Data indicate that the DNA helicase Rad5 ubiquitin ligase activity does not require ATP. PMID: 25690888
Findings suggest a structural role of the helicase domain of Rad5 and the helicase activity is dispensable for error-free lesion bypass. PMID: 24674630
A survey of various yeast deletion mutants converged on the Rad5 pathway of DNA damage tolerance by template switching as the likely target pathway of NSC109268 in mediating cellular sensitization to cisplatin. PMID: 24130896
Rad5 stably interacts with Rev1 via the N-terminal region of Rad5 and the C-terminal region of Rev1. PMID: 23142547
we genetically characterized a conserved yeast ICL repair pathway composed of the yeast homologs (Mph1, Chl1, Mhf1, Mhf2) of four FA proteins (FANCM, FANCJ, MHF1, MHF2 PMID: 22696213
The authors demonstrate that in the absence of PCNA SUMOylation, Mms2-Ubc13 and Rad5 may, independently of each other, function in the stimulation of translesion DNA synthesis. PMID: 21362066
Results indicate that multiple activities of Rad5 function coordinately with homologous recombination factors to enable replication template switch events that join sister chromatids at stalled forks and bypass DNA damage. PMID: 20541998
RAD5 contributes to DNA double-strand break repair via the Mre11/Rad50/Xrs2 protein complex. PMID: 16224103
In contrast to the role of Rad5 in postreplicational repair, however, where its function is coupled with that of Mms2-Ubc13, Rad5 function in translesion synthesis would be largely independent of this ubiquitin-conjugating enzyme complex PMID: 16908531
Data show that yeast Rad5 has a DNA helicase activity that is specialized for replication fork regression. PMID: 17936713
Rad5 is required for DNA polymerase zeta-dependent translesion synthesis in Saccharomyces cerevisiae PMID: 18757916
Rad5 acts both to align monoUb-PCNA with Ub-charged Ubc13 and to stimulate Ub transfer onto Lys(63) of a Ub acceptor. PMID: 19706603

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Subcellular Location

Cytoplasm. Nucleus.

Protein Families

SNF2/RAD54 helicase family

Database Links

KEGG: sce:YLR032W

STRING: 4932.YLR032W

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Recombinant Saccharomyces cerevisiae DNA repair protein RAD5 (RAD5), partial

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