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Recombinant Saccharomyces cerevisiae DNA replication regulator SLD3 (SLD3), partial

Recombinant Saccharomyces cerevisiae DNA replication regulator SLD3 (SLD3), partial

Code	CSB-YP345260SVG
MSDS	MSDS Datasheet
Size	Pls inquire
Source	Yeast
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Code	CSB-EP345260SVG
MSDS	MSDS Datasheet
Size	Pls inquire
Source	E.coli
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Code	CSB-EP345260SVG-B
MSDS	MSDS Datasheet
Size	Pls inquire
Source	E.coli
Conjugate	Avi-tag Biotinylated E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code	CSB-BP345260SVG
MSDS	MSDS Datasheet
Size	Pls inquire
Source	Baculovirus
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Code	CSB-MP345260SVG
MSDS	MSDS Datasheet
Size	Pls inquire
Source	Mammalian cell
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Product Details
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Customer Reviews and Q&A
Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

SLD3

Uniprot No.

P53135

Alternative Names

SLD3; YGL113W; G2980; DNA replication regulator SLD3

Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function

Required for loading and maintenance of stable association of CDC45 with chromatin during initiation and elongation of DNA replication. Also involved in temporal regulation of origin firing. Required for the association of PSF1 with origins.

Gene References into Functions

Here, the crystal structure of Sld7 in complex with Sld3 is presented. Sld7 comprises two structural domains. PMID: 26249346
The crystal structure of the central domain of Sld3 from Saccharomyces cerevisiae. PMID: 25126958
CDK substrates Sld3 and Sld2 and their binding partner Dpb11, together with the DDK subunit Dbf4 control S-phase length in budding yeast Saccharomyces cerevisiae limiting replication initiationst hrough their low abundance. PMID: 22081107
replication initiation protein Sld3 is phosphorylated by Rad53, and that this phosphorylation, along with phosphorylation of the Cdc7 kinase regulatory subunit Dbf4, blocks late origin firing in Saccharomyces cerevisiae PMID: 20865002
Sld3 does not move with DNA replication forks and only associates with MCM in an unstable manner before initiation. PMID: 16601689
Dpb11 connects phosphorylated Sld2 and Sld3 to facilitate interactions between replication proteins, such as Cdc45 and GINS PMID: 17167415
Sld2 and Sld3 are the minimal set of cyclin-dependent kinase targets required for DNA replication PMID: 17167417