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Recombinant Saccharomyces cerevisiae G1/S-specific cyclin CLN3 (CLN3), partial

Recombinant Saccharomyces cerevisiae G1/S-specific cyclin CLN3 (CLN3), partial

Code	CSB-YP318356SVG
MSDS	MSDS
Size	Pls inquire
Source	Yeast
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Code	CSB-EP318356SVG
MSDS	MSDS
Size	Pls inquire
Source	E.coli
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Code	CSB-EP318356SVG-B
MSDS	MSDS
Size	Pls inquire
Source	E.coli
Conjugate	Avi-tag Biotinylated E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code	CSB-BP318356SVG
MSDS	MSDS
Size	Pls inquire
Source	Baculovirus
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Code	CSB-MP318356SVG
MSDS	MSDS
Size	Pls inquire
Source	Mammalian cell
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Product Details
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Customer Reviews and Q&A
Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

CLN3

Uniprot No.

P13365

Alternative Names

CLN3; DAF1; WHI1; YAL040C; FUN10; G1/S-specific cyclin CLN3

Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function

Essential for the control of the cell cycle at the G1/S (start) transition. CLN3 may be an upstream activator of the G1 cyclins which directly catalyze start.

Gene References into Functions

G0/G1 transition, via Rim 15 and CLN3, might govern the fermentation rate of bottom-fermenting brewer's yeast in high-gravity wort. PMID: 27212268
Whi5 phosphorylation by Cln3-Cdk1 embedded in the G1/S network dynamically controls critical cell size and cell fate. PMID: 27094800
In CLN3, Whi3 may bind to the 3' GCAU motifs to attract the Ccr4-Not complex to promote RNA deadenylation and turnover, and Whi3 may bind to the 5' GCAU motifs to inhibit translation. PMID: 24386402
T36 phosphorylation triggers displacement of Ydj1, allowing Ssa1 to bind the G1 cyclin Cln3 and promote its degradation; these results establish an active role for Hsp70 chaperones as signal transducers mediating growth control of G1 cyclin abundance and activity PMID: 23217712
The spatial regulation of GAL1 and CLN3 transcripts is what allows growth to be maintained during fluctuations of glucose availability. PMID: 22160690
Cln3 regulates SBF by at least two different pathways, one involving the repressive protein Whi5, and the second involving Stb1 PMID: 19823669
Cln3 must overcome Far1 to trigger Cln-Cdc28 activation, which then turns on transcription; a second threshold is required together with the Cln3/Far1 threshold for carbon source modulation of protein content per cell at the onset of DNA replication PMID: 15520229
HPD(hydrophobic patch domain) of Cln3 identified important for interaction of Cln3 with Cdc28. PMID: 16200502
Cln3 contains a chaperone-regulatory Ji domain that counteracts Ydj1, a J chaperone essential for ER release and nuclear accumulation of Cln3 in late G1. PMID: 17560371
Cln3 activates only G1/S phase genes PMID: 19158491