Recombinant Saccharomyces cerevisiae Histone H1 (HHO1)

Code CSB-YP346469SVG
MSDS
Size Pls inquire
Source Yeast
Have Questions? Leave a Message or Start an on-line Chat
Code CSB-EP346469SVG
MSDS
Size Pls inquire
Source E.coli
Have Questions? Leave a Message or Start an on-line Chat
Code CSB-EP346469SVG-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
Have Questions? Leave a Message or Start an on-line Chat
Code CSB-BP346469SVG
MSDS
Size Pls inquire
Source Baculovirus
Have Questions? Leave a Message or Start an on-line Chat
Code CSB-MP346469SVG
MSDS
Size Pls inquire
Source Mammalian cell
Have Questions? Leave a Message or Start an on-line Chat

Product Details

Purity
>85% (SDS-PAGE)
Target Names
HHO1
Uniprot No.
Alternative Names
HHO1; YPL127C; LPI17C; Histone H1
Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Expression Region
1-258
Target Protein Sequence
MAPKKSTTKT TSKGKKPATS KGKEKSTSKA AIKKTTAKKE EASSKSYREL IIEGLTALKE RKGSSRPALK KFIKENYPIV GSASNFDLYF NNAIKKGVEA GDFEQPKGPA GAVKLAKKKS PEVKKEKEVS PKPKQAATSV SATASKAKAA STKLAPKKVV KKKSPTVTAK KASSPSSLTY KEMILKSMPQ LNDGKGSSRI VLKKYVKDTF SSKLKTSSNF DYLFNSAIKK CVENGELVQP KGPSGIIKLN KKKVKLST
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

 Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Target Background

Function
Could act as an H1-type linker histone. Has been shown to bind DNA.
Gene References into Functions
  1. data suggest that complete absence of Hho1 from chromatin does not affect reciprocal recombination between homologous chromosomes during meiosis PMID: 26122007
  2. deletion of HHO1 gene in S. cerevisiae leads to alterations in the higher-order levels of chromatin organization. PMID: 22200500
  3. Hho1 may play a dual role during sporulation: Hho1 and Ume6 depletion facilitates the onset of meiosis via activation of Ume6-repressed early meiotic genes, whereas Hho1 enrichment in mature spores contributes to spore genome compaction. PMID: 22586276
  4. findings demonstrated that the two globular domains independently associated with two different four-way junction molecules in a manner that required amino acid residues implicated in structure-specific binding in the nucleosome PMID: 16342967
  5. Hho1p reinforces the action of several types of barrier elements, and also inhibits silencing on its own. PMID: 16582449
  6. results suggest that histone H1 represses recombination at the rDNA by a mechanism that is independent of the recombination pathways regulated by Sir2 PMID: 18179596
  7. Hho1p is involved in rDNA compaction, and like core histones, is required for efficient rDNA transcription by pol I PMID: 18687885
  8. Hho1p has a limited role in transcriptional regulation; this dynamically exchanging linker histone may be evicted from chromatin by transcriptional activity. PMID: 18799740
  9. mode of Hho1p action is similar to that of canonical linker histones PMID: 19017647

Show More

Hide All

Subcellular Location
Nucleus. Chromosome.
Protein Families
Histone H1/H5 family
Database Links

KEGG: sce:YPL127C

STRING: 4932.YPL127C

icon of phone
Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
icon of address
Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
icon of social media
Join us with

Subscribe newsletter

Leave a message

* To protect against spam, please pass the CAPTCHA test below.
CAPTCHA verification
© 2007-2024 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1