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Recombinant Saccharomyces cerevisiae Increased sodium tolerance protein 2 (IST2), partial

Recombinant Saccharomyces cerevisiae Increased sodium tolerance protein 2 (IST2), partial

Code	CSB-YP327804SVG
MSDS	MSDS
Size	Pls inquire
Source	Yeast
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Code	CSB-EP327804SVG
MSDS	MSDS
Size	Pls inquire
Source	E.coli
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Code	CSB-EP327804SVG-B
MSDS	MSDS
Size	Pls inquire
Source	E.coli
Conjugate	Avi-tag Biotinylated E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code	CSB-BP327804SVG
MSDS	MSDS
Size	Pls inquire
Source	Baculovirus
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Code	CSB-MP327804SVG
MSDS	MSDS
Size	Pls inquire
Source	Mammalian cell
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Product Details
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Customer Reviews and Q&A
Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

IST2

Uniprot No.

P38250

Alternative Names

IST2; YBR086C; YBR0809; Increased sodium tolerance protein 2

Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function

May be involved in ion homeostasis together with BTN1 or BTN2.

Gene References into Functions

The authors show that, in BY4741 background, the lack of Ist2 results in the accumulation of higher amounts of sodium when the cells are exposed to the presence of this cation, demonstrating the importance of Ist2 for the maintenance of low intracellular levels of toxic sodium. PMID: 28199631
IST2 localization depends on the presence of a PM-binding domain, an intrinsically disordered linker region of sufficient length. PMID: 25409870
Data suggest that Btn2p, along with Btn1 and Ist2, may play a regulatory role across the cell in response to alterations in the intracellular environment. PMID: 15701790
Based on these observations, we suggest a local synthesis of Ist2p at cortical ER sites, from where the protein is sorted by a novel mechanism to the plasma membrane. PMID: 15911878
posttranslational mechanism, which leads to the concentration of Ist2--via multimerization--at ER sites, followed by direct transport to the plasma membrane PMID: 17234190
Results suggest that a direct interaction of the Ist2 sorting signal with lipids at the plasma membrane places Ist2 at contact sites between cortical ER and plasma membrane. PMID: 19208765
Data show that binding of phosphatidylinositol phosphates leads to efficient accumulation of Ist2 at domains of the cortical ER from where the protein may reach the PM independently of the function of the sec-pathway. PMID: 19453974

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Subcellular Location

Cell membrane; Multi-pass membrane protein. Note=Correct localization requires BTN2. Localizes to the mother cell in small budded cells and to the bud in medium and large budded cells. Transported to the bud tip by an actomyosin based process. Compartmentalization maintained by a septin mediated membrane diffusion barrier at the mother-bud neck.

Database Links

KEGG: sce:YBR086C

STRING: 4932.YBR086C

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